Analytical detection and method development of anticancer drug Gemcitabine HCl using gold nanoparticles
A simple, rapid, cost effective and extractive UV spectrophotometric method was developed for the
determination of Gemcitabine HCl (GMCT) in bulk drug and pharmaceutical formulation. It was based
on UV spectrophotometric measurements in which the drug reacts with gold nanoparticles (AuNP) and
changes the original colour of AuNP and forms a dark blue coloured solution which exhibits absorption
maximum at 688 nm. The apparent molar absorptivity and Sandell’s sensitivity coefficient were found to
be 3.95
×
10?5 l mol?1 cm?1 and 0.060 g cm?2 respectively. Beer’s law was obeyed in the concentration
range of 2.0–40 g ml?1. This method was tested and validated for various parameters according to ICH
guidelines. The proposed method was successfully applied for the determination of GMCT in pharmaceutical
formulation (parental formulation). The results demonstrated that the procedure is accurate, precise
and reproducible (relative standard deviation <2%). As it is simple, cheap and less time consuming, it can
be suitably applied for the estimation of GMCT in dosage forms.