AlphaThera Update: Fall edition ??
AlphaThera
UPenn spin-off biotech company enabling simple, fast and site-specific antibody conjugation for research and assays.
As we move closer towards the end of the year, we're excited to share new product updates, key progress and milestones achieved over the past few months.
Product Launches
Introducing: oYo-Link? SureLight? Fluorescent Proteins
For site-specific, covalent conjugation of primary antibodies with PE or APC in just 2 steps - mix & illuminate!
Choose from PE or APC labels. Discover more and order online here.
Conjugation services for as little as $75 per conjugate + the cost of reagents
Affordable, high quality antibody conjugation performed by our expert team
For just $75 per conjugate plus the cost of reagents, our expert in-house team will covalently and site-specifically label your antibody, ensuring you receive the highest quality conjugates that meet your specific experimental requirements.
FPLC Purification is also available post conjugation for $250 per conjugate.
Choose from our range of labels including oligos, drugs, biotin, enzymes and more.
Visit our website to find out more and chat to us about your project.
Our Customers' Work Featuring oYo-Link?
We're pleased to share some of our customers' work featuring oYo-Link?.
High-throughput, multiplexed quantification, and sorting of single EVs at single-molecule level
Here the authors present the BDEVS platform, which enables rapid, ultra-sensitive profiling of single extracellular vesicles (EVs) in plasma, detecting specific cancer and immune markers with single-molecule accuracy.
By combining amplification and flow cytometry, BDEVS achieves 100 times greater detection sensitivity than traditional methods, offering precise EV analysis for potential use in cancer diagnostics and monitoring, especially for tracking tumor-related immune responses in melanoma.
To ensure a constant number of primers per antibody, the authors used oYo-Link? Oligo to conjugate DNA oligomer to the antibody Fc region. Using oYo-Link? the researchers were able to quantify the number of biomarkers expressed on single EV based on the number of RCA products per agarose bead.
Multiplexed In Situ Imaging of Site-Specific m6A Methylation with Proximity Hybridization Followed by Primer Exchange Amplification (m6A-PHPEA)
The authors aimed to develop and demonstrate a novel method, termed m6A-PHPEA, for high-resolution, site-specific imaging of N6-methyladenosine (m6A) methylation at the single-cell level, enabling improved understanding of its regulatory roles and advancing epitranscriptomic research and diagnostics.
oYo-Link? DBCO was used to facilitate the conjugation of the azide-labeled DNA strand to the anti-m6A antibody through a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction, enabling efficient and biocompatible linkage between the DNA and the antibody for use as a recognition probe.?
领英推荐
Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging
In this study the researchers present a novel imaging method for rapid multiplexed super-resolution microscopy of a nearly unlimited number of molecular targets by leveraging fluorogenic labeling in conjunction with Transient Adapter-mediated switching for high-throughput DNA-PAINT (FLASH-PAINT).
oYo-Link Oligo was used to bind primary antibodies for FLASH-PAINT imaging of normal and bulbous-tip cilia in RPE-pHSmo cells.
Deep-Tissue Spatial Omics: Imaging Whole-Embryo Transcriptomics and Subcellular Structures at High Spatial Resolution
This study introduces cycleHCR, a technology leveraging multicycle DNA barcoding and Hybridization Chain Reaction (HCR) to surpass the inherent limitations of fluorescence microscopy that constrain comprehensive spatial analysis in biological specimens. cycleHCR facilitates high-specificity, single-shot imaging per target for RNA and protein species within thick specimens, mitigating the molecular crowding issues encountered with other imaging-based spatial omics techniques.
To harness the high specificity of cycleHCR for multiplex protein imaging, the researchers used oYo-Link? Oligo to form an antibody complex that simultaneously recognizes the target and anchors a cycleHCR barcode within a polyacrylamide gel for imaging readout.
DNA-based AND logic gate as a molecular precision tool: selective recognition of protein pairs in lipid nanodiscs and subsequent binding of gold nanorods
This study introduces a DNA-based molecular logic AND gate designed to recognize the two membrane proteins PD-L1 and CD3 as antigens via corresponding antibody-oligonucleotide conjugates. To enable the DNA logic gate to selectively target the desired pair of membrane proteins out of a reaction mixture, the researchers used oYo-Link? Oligo to covalently link input strands to monoclonal antibodies, and the resulting DNA-antibody conjugates were implemented into the construct as targeting agents.
Epitope Tag Conjugation system provides versatility for multiples assay design
When running a multiplex assay researchers run the risk of reduced activity and specificity of conjugated antibodies. In this poster video, Fortis Life Sciences present their approach to developing an easy and adaptable system for generating conjugated antibodies to use in multiplex assays.
Using AlphaThera’s oYo-Link? Epitope Tag conjugation system they were able to retain conjugate specificity and activity enabling development of a highly versatile multiplex assay system using Fortis' anti-epitope tag detection antibodies.
They used this system to detect staining of multiple markers including CD3, Granzyme B, PCNA, and actin via flowcytometry, IHC, ICC, and western blot.
Download the poster here.
Find out more about oYo-Link Epitope Tags here.
Happy Thanksgiving from the AlphaThera team! ????
Wishing all our US colleagues and customers a Happy Thanksgiving. We hope you have a wonderful holiday with your family and loved ones.
If you have any questions about the content of this newsletter or about our oYo-Link? technology and conjugation services, please don’t hesitate to contact us.