Advancements in Nanoparticle Tracking Analysis: A New Era for EV Research

Introduction?

Extracellular Vesicles (EVs) are tiny particles released by cells that play crucial roles in various biological processes. Understanding their characteristics and functions is essential for advancements in medical and biological research. One of the key techniques for analysing EVs is Nanoparticle Tracking Analysis (NTA). Recently, significant advancements have been made in this field, particularly with the introduction of fluorescence-based NTA (F-NTA).?

The Evolution of NTA?

Traditional NTA has been a cornerstone in EV research, allowing scientists to measure the size and concentration of these particles. However, the introduction of F-NTA has revolutionised the field by enabling targeted analysis through fluorescence detection. This advancement has significantly improved the detection efficiency of specific EV subpopulations.?

Introducing? ZetaView??

The latest innovation in NTA technology is the Particle Metrix ZetaView? x30 system. The instrument stands out because it allows for the detection of two independent fluorochromes on the same particle. This capability is crucial for colocalization measurements, where researchers can determine if two different markers are present on the same EV.?

The ZetaView? system stands out from outdated NTA instruments with its advanced fluorescence-based NTA (F-NTA) technology, which brings several key advantages:?

1. Dual Fluorochrome Detection: The ZetaView? PMX-230 TWIN & QUATT allows for the detection of two independent fluorochromes on the same particle. This capability is essential for colocalization measurements, enabling researchers to determine if two different markers are present on the same EV.?
2. Enhanced Detection Efficiency: With F-NTA, the ZetaView? system significantly improves the detection efficiency of specific EV subpopulations. This targeted analysis is crucial for understanding the complex roles of EVs in various biological processes.?
3. Reliable and Comparable Data: The ZetaView? system’s results are comparable with other advanced methodologies, such as imaging flow cytometry. This ensures that researchers can trust the data they obtain, making it a reliable tool for EV analysis.?

Detection and measurement of CD9 and CD41 on double-stained human platelet derived MSC-EV preparations?

In a recent study, researchers demonstrated that fluorescence-based NTA (F-NTA) is an effective method for identifying human platelet-derived MSC-EVs in a complex mix of biological nanoparticles. The method is fast, reproducible, and does not require a cleaning step to remove unbound antibodies. Colocalization results from F-NTA and imaging flow cytometry (IFCM) were highly comparable, confirming the presence of CD41-positive and CD9-positive EVs. F-NTA provided more specific detection of EVs compared to scatter-based NTA, and colocalization NTA (C-NTA) proved accurate for single-particle biomarker detection and size determination. C-NTA complements F-NTA and scatter-based NTA, offering more targeted and detailed analysis of extracellular vesicles.?

Read the full study here

Applications Across Various Fields?

The ZetaView? system is versatile and can be applied in numerous research areas, including:?

• Disease Diagnosis: Detect disease-specific markers in biological fluids for conditions like cancer and cardiovascular diseases.?

• Therapeutic Development: Monitor the stability and composition of therapeutic EVs to ensure efficacy and safety.?

• Cancer Research: Study the mechanisms of tumour growth, metastasis, and drug resistance.?

• Cardiovascular and Neurodegenerative Research: Understand the roles of EVs in diseases like atherosclerosis, stroke, Alzheimer’s, and Parkinson’s.?

• Regenerative Medicine: Optimize the use of stem cell-derived EVs for tissue repair and regeneration.?

?Conclusion?

The advancements in NTA, particularly with the introduction of the Particle Metrix ZetaView? system, represent a significant leap forward in EV research. These technologies not only enhance our understanding of EVs but also open new avenues for their application in various biological and medical fields. As research continues to evolve, the potential for new discoveries and innovations in EV analysis remains vast.?

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Join Our Training Sessions?

At Analytik, we are committed to supporting our customers every step of the way. We offer comprehensive training sessions to help you get the most out of your ZetaView? system. Our expert team will guide you through the setup, operation, and data analysis, ensuring you can fully leverage the capabilities of F-NTA technology.?

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Contact Us Today?

Ready to take your EV research to the next level? Contact Analytik today to learn more about the ZetaView? system and schedule your training session. Discover how this advanced technology can transform your research and lead to groundbreaking discoveries.?

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