11 CAM Assay Publications to advance your research ????
We hope this newsletter finds you well and brimming with curiosity! We present to you a collection of our publications centered around the fascinating CAM Assay ???? using our AI application.
Our Chorioallantoic Membrane Assay application, or CAM Assay app, has been a cornerstone in our endeavors. It offers a unique window into angiogenesis, tumor growth, and developmental biology. Here are some high-ranking biomedical publications that have already featured the IKOSA CAM Assay app.?
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The CAM Model—Q&A with Experts
Summary: The chick chorioallantoic membrane (CAM) assay is an alternative in vivo model for cancer research, offering rapid and cost-effective tumor growth, drug response, and angiogenesis studies. While not as established as mouse models, CAM's value in cancer research is continuously growing.
Authors: Dagmar Fischer (ResearchGate), Prof. Dr. med. Georg Fluegen , Paul Garcia , Nassim Ghaffari Tabrizi-Wizsy , Laura Gribaldo , Ruby Yun-Ju Huang , Volker Rasche , Domenico Ribatti (ResearchGate), Xavier Rousset , Marta Teixeira Pinto, PhD , Jean Viallet , Yan WANG , and Regine Schneider-Stock .
Institutions: FAU Erlangen-Nürnberg , Medizinische Universit?t Graz , INOVOTION , 台湾大学 , Universit?t Ulm , Università degli Studi di Bari , i3S - Instituto de Investiga??o e Inova??o em Saúde
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Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken’s Chorioallantoic Membrane (CAM Assay)
Summary: The CAM assay employs fertilized hen's eggs as an animal experimentation platform, addressing animal welfare concerns and potential limitations of rodent models. It assesses CAM irritation, organ damage, embryo mortality, and supports xenograft implantation. With in vivo microscopy and imaging techniques, it provides insights into toxicology and angiogenesis. Ethical considerations, affordability, and minimal bureaucracy validate its use.
Authors:?Nadine Wiesmann (ResearchGate), Jürgen Brieger (ResearchGate) and Dr. med. Jonas Eckrich (ResearchGate)
Institution: Universit?tsmedizin Mainz
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IKOSA? CAM Assay Application to Quantify Blood Vessels on Chick Chorioallantoic Membrane (CAM)
Summary: The CAM Assay offers a cost-effective and ethically viable approach to studying angiogenesis. A segmentation method using IKOSA CAM Assay enables quantification of blood vessel-related processes. This involves analyzing in vivo CAM images to measure attributes like vascular area, length, thickness, and branching points. The chapter outlines the assay procedure and details the IKOSA CAM Assay outcomes.
Authors: Tiziana Annese (ResearchGate), Roberto Tamma (ResearchGate) and Domenico Ribatti (ResearchGate)
Institution: Università degli Studi di Bari
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Deep Learning-Based Image Analysis for the Quantification of Tumor-Induced Angiogenesis in the 3D In Vivo Tumor Model—Establishment and Addition to Laser Speckle Contrast Imaging (LSCI)
Summary: This study enhances angiogenesis analysis using the CAM assay application on the IKOSA platform. Employing deep learning and laser speckle contrast imaging, angiosarcoma tumors were studied on the CAM. Perfusivity increased with both methods, showing CAM assay application's validity. Gluconate exhibited anti-angiogenic effects, offering potential against angiosarcomas. The CAM assay application holds promise for angiogenesis quantification and gluconate treatment assessment, warranting further investigation.
Authors: Paulina Mena Kuri (ResearchGate), Eric Pion (ResearchGate), Lina Mahl (ResearchGate), Philipp Kainz (ResearchGate), Siegfried Schwarz , Christoph Brochhausen (ResearchGate), Thiha Aung (Publications) and Silke H?rteis (ResearchGate).
Institutions: Universit?t Regensburg and Technische Hochschule Deggendorf
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A Novel Artificial Intelligence-Based Approach for Quantitative Assessment of Angiogenesis in the Ex Ovo CAM Model
Summary: Angiogenesis, crucial for tissue regeneration and tumor growth, is targeted by diverse therapies. Quantifying vasculature is vital for angiogenesis research. The CAM assay utilizes ex ovo cultured chick embryos to develop a vascularized membrane for studying cancer angiogenesis. Four methods were compared for CAM vasculature quantification, revealing the IKOSA CAM assay application as optimal. This reliable software aids practical angiogenesis research, enabling deeper exploration and potential anti-angiogenic cancer treatment strategies.
Authors: Lorenz F. , Bardia Firouz, Paul Slezak (ResearchGate), Cyrill Slezak (ResearchGate), Michael Wei?ensteiner , Thomas Ebner (ResearchGate), Nassim Ghaffari Tabrizi-Wizsy (ResearchGate), Kurt Schicho (ResearchGate) and Peter Dungel (ResearchGate).
Institutions: Medizinische Universit?t Wien , Utah Valley University , Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA , and Medizinische Universit?t Graz
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The Chick Embryo Xenograft Model for Malignant Pleural Mesothelioma: A Cost and Time Efficient 3Rs Model for Drug Target Evaluation
Summary: Malignant pleural mesothelioma (MPM), often linked to asbestos exposure, has limited treatment success due to genetic diversity. A novel preclinical model using fertilized hen’s eggs was created. This model efficiently grows MPM cells on the chick chorioallantoic membrane (CAM), enabling monitoring through fluorescence and bioluminescence imaging. Ten MPM cell lines with varying genetic profiles were tested, and the CAM model mimicked key features of the disease. Bioluminescence imaging accurately gauged tumor size and viability, correlating with histological analysis. This versatile, cost-effective approach provides insights into MPM diversity and drug evaluation, potentially replacing rodent models.
Authors: Sarah Barnett (ResearchGate), Anne Herrmann (ResearchGate), Liam Shaw, Non Gash , Harish Poptani (ResearchGate),?Joseph Sacco (ResearchGate) and Judy Coulson (ResearchGate).
Institution: University of Liverpool
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A 3D In Vivo Model for Studying Human Renal Cystic Tissue and Mouse Kidney Slices
Summary: Autosomal dominant polycystic kidney disease (ADPKD) causes kidney cysts and failure. Clinical trials for cyst growth inhibition often fail due to inadequate models. This study established a CAM-based model using renal tissue from ADPKD patients and mouse kidneys. Tissues were cultured on the CAM, showing cyst growth over a week, correlating with weight and angiogenesis. The model offers a bridge between animal studies and clinical trials, providing a platform for drug testing and insights into cyst enlargement and renal physiology without extensive animal experiments.
Authors: Eva-Marie Bichlmayer (ResearchGate), Lina Mahl (ResearchGate), Leo Hesse, Eric Pion (ResearchGate), Victoria Haller, Andreas Moehwald, Christina Hackl (ResearchGate), Jens M. Werner (ResearchGate), Hans Schlitt (ResearchGate), Philipp Kainz (ResearchGate), Siegfried Schwarz , Christoph Brochhausen (ResearchGate), Christian Groeger, Felix Steger, Oliver K?lbl (ResearchGate), Christoph Daniel (ResearchGate), Kerstin Amann (ResearchGate), Andre Kraus (ResearchGate), Bj?rn Buchholz (ResearchGate), Thiha Aung (Publications) and Silke H?rteis (ResearchGate).
Institutions: Universit?t Regensburg , Universit?tsklinikum Regensburg , FAU Erlangen-Nürnberg and Technische Hochschule Deggendorf
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Isolation and characterization of ZK002, a novel dual function snake venom protein from Deinagkistrodon acutus with anti-angiogenic and anti-inflammatory properties
Summary: ZK002, a novel snake venom-derived protein, exhibits anti-angiogenic and anti-inflammatory properties, making it a promising therapeutic candidate for diseases involving angiogenesis and inflammation, such as cancer, diabetic retinopathy, psoriasis, arthritis, and inflammatory bowel disease. Further research is needed to fully establish ZK002's safety and efficacy in humans.
Authors: Brandon Dow Chan, Wing-Yan Wong (ResearchGate), Magnolia Muk-Lan Lee (ResearchGate), Patrick Yue, Xiangrong Dai (戴向荣), Karl Tsim, Wen-Luan Wendy Hsiao (ResearchGate), Mandy Li, Xiao-Yi Li, William Chi-Shing Tai
Institutions: The Hong Kong Polytechnic University, Hong Kong Baptist University,Lee's Pharmaceutical Holdings Limited, The Hong Kong University of Science and Technology, 澳門科技大學, and Zhaoke Ophthalmology
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Hyaluronic Acid with Bone Substitutes Enhance Angiogenesis In Vivo
Summary: The study aimed to evaluate the pro-angiogenic properties of a commercially available bovine bone-substitute material with hyaluronic acid (BSM+) compared to the same material without hyaluronic acid (BSM). Results revealed that BSM+ significantly enhanced blood vessel formation compared to BSM, suggesting its potential to accelerate bone regeneration and healing.
Authors: Solomiya Kyyak , Sebastian Blatt (ResearchGate), Nadine Wiesmann (ResearchGate), Prof. Dr. Dr. Ralf Smeets , and Peer W. K?mmerer
Institutions: Johannes Gutenberg-Universit?t Mainz and Universit?tsklinikum Hamburg Eppendorf
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Zinc Oxide Nanoparticles Exhibit Favorable Properties to Promote Tissue Integration of Biomaterials
Summary: Zinc oxide nanoparticles (ZnO NPs) exhibit promising properties for tissue engineering applications, demonstrating biocompatibility, antibacterial effects, and the ability to enhance metabolic activity of key cells involved in implant integration. Their incorporation into biomaterials could promote complication-free healing and pave the way for advanced tissue engineering approaches.
Authors: Nadine Wiesmann (ResearchGate), Simone Mendler, Christoph Raphael Buhr , Ulrike Ritz , Peer W. K?mmerer, and Jürgen Brieger (ResearchGate)
Institutions: Johannes Gutenberg-Universit?t Mainz
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Analysis of Osteosarcoma Cell Lines and Patient Tissue Using a 3D In Vivo Tumor Model—Possible Effects of Punicalagin
Summary: Punicalagin shows promise as a supplementary treatment for osteosarcomas, reducing tumor weight, proliferation, and angiogenesis in an in vivo model. It could offer hope for improved outcomes in patients with metastatic disease.
Authors: Anna Rebecca Dorn, Sara Neff, Sophia Hupp, Melissa Engelhardt, Eric Pion, Ulrich Lenze, PD Dr. med. Carolin Knebel, PD Dr.med. Anna Duprée, Dr. med. Simone Schewe, Markus Weber, Christian Wulbrand, Axel Prof. Dr. Hillmann, Florian Weber, Phillip Clarke, Philipp Kainz, T. AUNG, and Silke H?rteis
Institutions: University of Regensburg, Technical University of Munich, Universit?tsklinikum Hamburg Eppendorf, Hospital Barmherzige Brüder Regensburg, and Deggendorf Institute of Technology
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