2024 Updated mRNA Purification Methods

Purification, a crucial step in mRNA production, involves removing impurities such as unreacted chemicals or residual materials.?

The?purification?process?ensures?batch-to-batch consistency of mRNA and lipid?nanoparticle?(LNP)?formulations,?removing?unstable?components?and?thereby improving?their?bioavailability?and?effectiveness?in?vivo.

Here are several common purification methods:

1. Lithium chloride precipitation: RNA forms complexes with lithium chloride and alcohol, precipitating out, followed by centrifugation to separate RNA precipitate for purification;

2. Magnetic bead purification: Surface-modified magnetic beads selectively bind to RNA, allowing rapid and efficient purification via controlled movement with a magnetic field;

3. Affinity chromatography: Utilizing the affinity between mRNA polyA and oligo dT to capture RNA and remove impurities;

4. Ion exchange chromatography: Leveraging differences in interactions between RNA and ion exchange groups, separation and purification of RNA are achieved by adjusting ion strength and pH in the ion exchange column.

Relied on our powerful and proprietary RNASci platform, we also provide bespoke RNA synthesis services, as well as mRNA (up to 10 kb), circRNA (up to 4 kb) and LNP catalogue products. We have established a complete set of mRNA/circRNA synthesis technologies to provide high-quality RNA, such as sequence design and optimization, IVT, circularization, purification, and LNP encapsulation. All products are released under the stringent quality control standards.

If this article has got you interested in purification, please feel free to contact Yaohai Bio-pharma. We will provide you with the customized services.

Yaohai?Bio-Pharma is also actively seeking institutional or individual global partners and offers the most competitive compensation in the industry. If you have any questions,please feel free to contact us: [email protected]

For more details about Yaohai Bio-Pharma, please kindly refer to our website: www.yaohaibio-pharma.com