Mychel Raony Paiva Teixeira Morais的动态

Excited to share our latest work, a product of collaboration between three talented UVA MSTP students Jeffrey Hsu, Kimberly Nguyen & Magda Bujnowska, and Kevin Janes lab: An easy-to-follow protocol for highly-multiplexed iterative indirect immunofluorescence imaging (4i) in cultured cells, formalin-fixed paraffin-embedded (FFPE) tissue sections, and chromosome spreads. In this paper, we detail procedures for sample preparation, antibody and DNA staining, immunofluorescence imaging, antibody elution, and image processing. This protocol is adapted for high-throughput analysis of fixed cultured cells and addresses sample-specific challenges such as intrinsic tissue autofluorescence and chromosome fragility. https://lnkd.in/eeUDAJFG

Happy to share our protocol for mapping physiological DSBs by in-suspension break labeling in situ and sequencing (sBLISS) published in cell press’ STAR Protocols. This protocol was used to study how transcriptional DSBs are formed, uncovering the important role of R-loops and TOP1cc in the formation of these critical DSBs. Check out our paper for more details on how this protocol was implemented: https://lnkd.in/dEutCSjb In this protocol, we explain in detail the step-by-step experimental procedure as well as the data analysis and dive into why we favored this method over others as well as its limitations. All thanks and gratefulness for our prof Rami Aqeilan for his endless opportunities, guidance, and supervision. Check out our protocol here: https://lnkd.in/dHK6SVv6

Molecular Beacon probes are dual-labelled probes that form a stem-loop (hairpin) structure, bringing the reporter and quencher into proximity. The loop region contains the sequence that hybridises to the target sequence, while the complementary sequences at both ends of the probe form the stem. These probes generate fluorescence under non-hydrolytic conditions via hybridisation to the target. Learn more in the video below: https://hubs.li/Q02q8qPJ0

Unlock the potential of magnetic beads for biomolecule isolation and purification with Cytiva. Our reliable and innovative methods simplify the process and boost consistency, so you can increase success rates. Read the article to learn how. https://cytiva.link/ilfbf #magneticbeads #diagnostics

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Gain precise molecular insights using radiometric detection. Transform #drugdiscovery & development with unmatched sensitivity. Explore further. https://ms.spr.ly/6046YWTja #radiometricdetector #drugdevelopment

Gain precise molecular insights using radiometric detection. Transform #drugdiscovery & development with unmatched sensitivity. Explore further. https://ms.spr.ly/6044YWTo0 #radiometricdetector #drugdevelopment