Excited to share our latest work, a product of collaboration between three talented UVA MSTP students Jeffrey Hsu, Kimberly Nguyen & Magda Bujnowska, and Kevin Janes lab: An easy-to-follow protocol for highly-multiplexed iterative indirect immunofluorescence imaging (4i) in cultured cells, formalin-fixed paraffin-embedded (FFPE) tissue sections, and chromosome spreads. In this paper, we detail procedures for sample preparation, antibody and DNA staining, immunofluorescence imaging, antibody elution, and image processing. This protocol is adapted for high-throughput analysis of fixed cultured cells and addresses sample-specific challenges such as intrinsic tissue autofluorescence and chromosome fragility. https://lnkd.in/eeUDAJFG
Mohammad Fallahi-Sichani的动态
最相关的动态
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?? ????????'?? ???????? ?????????????? ?????????????? (#??) - New Advances in Extracellular Vesicle miRNA Research Using High-Throughput Affinity Capture ?? ?? EV miRNA research just got an upgrade. It’s like giving a magnifying glass to Sherlock Holmes, but for extracellular vesicles – and way cooler. ?? Don’t miss out to watch on-demand the latest findings: https://bit.ly/4iNvXIw
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Happy to share our protocol for mapping physiological DSBs by in-suspension break labeling in situ and sequencing (sBLISS) published in cell press’ STAR Protocols. This protocol was used to study how transcriptional DSBs are formed, uncovering the important role of R-loops and TOP1cc in the formation of these critical DSBs. Check out our paper for more details on how this protocol was implemented: https://lnkd.in/dEutCSjb In this protocol, we explain in detail the step-by-step experimental procedure as well as the data analysis and dive into why we favored this method over others as well as its limitations. All thanks and gratefulness for our prof Rami Aqeilan for his endless opportunities, guidance, and supervision. Check out our protocol here: https://lnkd.in/dHK6SVv6
Cell Press: STAR Protocols
star-protocols.cell.com
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2. Patrick Coulombe just published in Nature Communications through bar coding studies, convincing proof that UM171, the KBTBD4-HDAC2 molecular glue discovered by our group and that of Anne Marinier at IRIC, treated HSCs self-renew in ex vivo cultures. More interestingly, he showed that UM171 induces the expansion of several important progenitors, namely the EMMP which transient loose the ability to undergo mega and erythroid differentiation shifting to mast progenitors which lead to engratment in vivo. This work consolidate findings suggesting CoREST degraders such as UM171 may play an important role in strategies to treat MPN in which excessive platelet and red blood cell production occurs.
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Tips for quickly removing culture medium from 96-well plates. ?? Suitable for poor experimenters who don’t have a multichannel pipettor. ?? #Daily Lab #Cell Experiment #Scientific Research #Pipette Tips
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I really encourage scientists embarking on full spectral flow cytometry to take the time to listen to this excellent webinar from Bob Balderas and Florian Mair. There are so many additional considerations when performing spectral cytometry... so why not learn from the experts? #BDBiosciences #BDFACSDiscoverS8 #flowcytometrydyes #BDHorizon #flowcytometry
Missed the talk by Bob Balderas and Florian Mair on improving biological resolution and increasing parameters with spectral flow cytometry at #CYTO2024? Visit our CYTO2024 event page to watch the recorded video of the talk! You can also download the posters while you are there. https://lnkd.in/giZKqs8s #BDBiosciences #BDFACSDiscoverS8 #flowcytometrydyes #BDHorizon #flowcytometry
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Dive into the latest breakthrough in cellular analysis! Our application note explores the journey from Postmortem Homogeneous Cells to Microarray Analysis using the Accuva Cellect Laser-Capture Microdissection (LCM) System. Discover how this innovative technology revolutionizes precision in cell research.
Dive into the latest breakthrough in cellular analysis! Our application note explores the journey from Postmortem Homogeneous Cells to Microarray Analysis using the Accuva Cellect Laser-Capture Microdissection (LCM) System. Discover how this innovative technology revolutionizes precision in cell research. www.laxcoinc.com/lcm Read more: https://lnkd.in/gEUUCp5p ?#CellularAnalysis #LCMSystem #ResearchInnovation
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Traditional methods of assessing cell health and behaviour often rely on subjective observations and manual interventions, leading to variability and inefficiencies. Enter Agilent's BioTek LionHeart FX, that can revolutionise cell culture workflows by providing real-time, quantitative data on cell morphology, viability, and proliferation. BioTek Lionheart FX allows you to capture, process, analyse, annotate images, and produce videos with ease. Discover more about this instrument here: https://buff.ly/4aJGoIc #Chemetrix #BioTekLionHeart #AnalyticalInstrument #Laboratory #CellAnalysis #CellCulture
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Gain precise molecular insights using radiometric detection. Transform #drugdiscovery & development with unmatched sensitivity. Explore further. https://ms.spr.ly/6045YWTgT #radiometricdetector #drugdevelopment
Radiometric detection for precise and efficient molecular quantitation
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The poster session is live! Come stop by at poster 946 and learn more about our platform technology!
Everyone's favorite T-cell scientist, Zachary Imam, PhD, is presenting poster #946 at #ISCT2024, "Expansion of Stem-Like T Cells by Protein Nanoscaffold-Based NanoSpark STEM-T Activator." He would love to walk you through his latest data on our soluble, best-in-class T-cell activator NanoSpark STEM-T! #celltherapy #Tcells #NKcells
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Gain precise molecular insights using radiometric detection. Transform #drugdiscovery & development with unmatched sensitivity. Explore further. https://ms.spr.ly/6044YWTo0 #radiometricdetector #drugdevelopment
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Associate Professor at National Tsing Hua University, studying heterogeneity, gene expression, and signaling in cancer
7 个月Very helpful!