BioSkryb Genomics的动态

RNA N6-adenine methylation dynamics impact?Hyaloperonospora arabidopsidis?resistance in?Arabidopsis Studying N6-adenine methylation (m6A) changes during the biotic stress response in?Arabidopsis?is challenging with immunoprecipitation techniques. To overcome these challenges, the authors used direct RNA nanopore sequencing to resolve exact m6A positions at single nucleotide resolution. The team discovered m6A changes during the defence response against the parasite,?H. arabidopsidis, and concluded that m6A could be a promising target to investigate for improved crop resistance against pathogens. https://lnkd.in/gPAp_EZ8

Characteristics and phylogenetic distribution of megaplasmids and prediction of a putative chromid in Pseudomonas aeruginosa. Read the article here: https://lnkd.in/gupAwGMX

Genome-wide association study and molecular marker development for susceptibility to Gibberella ear rot in maize https://lnkd.in/gpN8iiJQ

In my latest article, I highlight the importance of nucleic acid cleanup and its impact on achieving high-quality sequencing results and explain how my colleagues at Beckman Coulter Life Sciences and I can assist you every step of the way. https://lnkd.in/g6u_tsGr #Genomics #NGS #LabSolutions #BeckmanCoulterLifeSciences #NucleicAcidCleanup

Bacterial response to DNA damage and cell cycle regulation was paradigm around LaxA/RecA mediated SOS response while eukaryotic response was paradigm around ATM/ATR response and cascade of Ser/The phosphorylation/ dephosphorylation. This demarcation was linked to evolution and said that bacteria ( Esherichia coli) do not have stress responsive signaling STPKs albeit stress response was assigned to TCS. the genome sequence explosion has made us to revisit the logic of bacteria not having stress responsive STPKs. Research on Deinococcus radiodurans and literature survey with other bacteria have led us to strongly believe that bacteria can have STPK mediated DNA damage response and cell cycle regulation, very similar to eukaryotes. Deinococcus an extremely radio resistant bacterium has a DNA damage responsive Ser/Thr quinoprotein kinase (RqkA) that had shown most of the properties similar to ATM/ATR. The perspective article linked with this post might open a new dimension of research in days to come.

Save the date April 2, noon EST for the next CSMB Virtual Seminar featuring Dr. Sabri Rial (University of Montreal) – “The scaffold protein 14-3-3zeta and its interactome are required for expression of key adipogenic genes during the early stages of adipocyte differentiation” & Dr. Zoe Gillespie (University of Toronto) – “Sli-C: Adapting HiC to examine three-dimensional genome interactions in limited cell populations” https://buff.ly/3HyokUT

??????Whatsapp!! ??????Structure and repair of replication-coupled DNA breaks Using #CRISPR/Cas9 nicking #enzymes, we examine the interaction between the #replication machinery and single-strand breaks, one of the most common forms of endogenous #DNA damage. We show that #replication #fork collapse at leading strand nicks generates resected #single_ended #double_strand #breaks (seDSBs) that are repaired by homologous #recombination (HR). If these seDSBs are not promptly repaired, the arrival of adjacent forks creates double-ended DSBs (deDSBs), which could drive genomic scarring in HR-deficient cancers. deDSBs can also be generated directly when the replication fork bypasses lagging strand nicks. Unlike deDSBs produced independently of replication, #end-resection at nick-induced se/deDSBs is BRCA1-independent. Nevertheless, BRCA1 #antagonizes 53BP1 #suppression of RAD51 #filament #formation. These results highlight unique #mechanisms that maintain replication fork stability. ? ?? You can find more pieces of work by clicking here. https://lnkd.in/eSG67K5G https://lnkd.in/dB3s88ei #repair #replication #DNA #CRISPR #enzymes

Protein involved in balancing DNA replication and restarting found In a new paper published in?Nature Communications, an international team of researchers have found that the protein USP50 supports the DNA replication process by helping to decide the proper use of nucleases or helicases. These enzymes are implemented during the DNA replication process to promote ongoing replication and where the copying machinery runs into problems and needs to restart. https://lnkd.in/ezJcnpq8

This video of a bacteriophage (virus that infects a specific bacteria) is too cool not to share. This is what we need to fight bacteria infections. Bacteriophages are personalized medicine that works like a scalpel to get rid of just the bad bacteria while leaving the good. SOOO much better than antibiotics (fungal that kills ALL bacteria). Antibiotics are nuclear bombs killing one bad bacteria at the cost of all the good bacteria keeping you healthy. If we want to counter antibiotic resistance diseases the FDA needs to make procedures to obtain the right bad bacteria, remove things like endotoxins, storage and transport conditions and make the personalized medicine of bacteriophages the standard of care in this country. To watch a video click this link https://lnkd.in/g2v54Ehv or the link to "Steffanie" below in the box More info here https://lnkd.in/gSY6xsyw #bacteriophage #phage #CombatAntibioticResistance #FDA

Spliceosomes work hard to remove sequences called “introns" from genes before cells can make the proteins they need, but they don't stop there! After the spliceosome is finished splicing the mRNA, it remains active and can engage in further reactions with the removed introns. A new study by Manuel Ares reports on a surprising discovery about the spliceosome that could tell us more about the evolution of different species and the way cells have adapted to the strange problem of introns! Read more about it on news.ucsc.edu