MGI的动态

In recent years, #CRISPR Technology has emerged as a game-changer in the field of gene editing, gaining approval from FDA for treatment of sickle cell disease. With the evolution of CRISPR/CasX systems, there's a growing emphasis on developing ultra-long #sgRNA to address challenges like off-target effects and incomplete edits. Synthesizing long oligos exceeding 100 bases presents unique challenges, yet our team at Synoligo has mastered the art. We're proud to consistently deliver sgRNAs upto 200 bases with exceptional precision and scalability. Check out our data from the blog here: https://lnkd.in/eth_kJhm #geneediting #oligonucleotides #biopharma

Discover the power of the Casilio expression vectors for advanced gene editing. The Casilio system, a hybrid of CRISPR/dCas9 and Pumilio RNA-binding domains, offers distinct advantages over conventional CRISPR/Cas9 approaches. With the ability to regulate multiple target genes simultaneously, Casilio provides greater flexibility and precision for studying gene functions in living organisms. Explore how Casilio vectors can be used for gene editing, transcription regulation, and DNA-binding fluorescent protein detection, all while maintaining compatibility with your existing workflows. #GeneEditing #CRISPR

We are excited to share a recent publication highlighting the crucial role of geneXplain’s TRANSFAC database and MATCH algorithm in groundbreaking research. The study, titled "Ectopic MYBL2-Mediated Regulation of Androglobin Gene Expression," investigates the regulatory mechanisms controlling the expression of the Androglobin (ADGB) gene, a novel member of the globin superfamily with significant implications for spermatogenesis and ciliogenesis. Researchers utilized the TRANSFAC database to identify potential transcription factor binding sites within the ADGB promoter region. The MATCH algorithm was instrumental in pinpointing the specific binding sites for MYBL2 and PITX2, which were then validated through various experimental approaches, including luciferase reporter assays and chromatin immunoprecipitation (ChIP)-qPCR. This comprehensive analysis revealed that ectopic expression of MYBL2 significantly enhances ADGB mRNA levels, indicating a robust regulatory role. This research does not only enhance the knowledge about ADGB transcriptional regulation but also indicates to potential functional contexts beyond its established role. This study underscores the value of TRANSFAC and MATCH in elucidating complex gene regulatory networks, paving the way for deeper insights into gene expression and regulation. For more details, read the full publication?https://lnkd.in/edf8hway #TRANSFAC #geneXplain #genes #gene #protein #expression #TFBS #TranscriptionFactors #Bioinformatics #research #science #biology #Androglobin

“Single cell analysis takes us an extra step forward in understanding down to the actual number of cells that have these translocations, which is something bulk techniques cannot show.” At this year's ASGCT conference, Senior Director of Product Management Vanee Pho sat down with CGTLive to discuss the importance of detecting translocations caused by gene editing products and how Mission Bio’s technology can help address this need. Check out her full interview below ? https://lnkd.in/gyjVDqFZ #cgt #celltherapy #genetherapy #geneediting #genomeediting

Gene editing isn’t just about #CRISPR/Cas—though it’s undeniably a revolutionary tool!. I’m taking this opportunity to reflect on a recent study analyzing #LNPs formulated with four different ionizable lipids for delivering gene-editing RNPs. This raises an important question: ?? What do you consider the benchmark #ionizablelipid for gene editing? ?? With so much innovation in ionizable lipid design, it’s clear that different applications might require tailored solutions.

?? Dive into the world of CRISPR-Cas9 gene editing with our latest blog post! Discover how this groundbreaking technology is transforming clone screening and accelerating scientific breakthroughs. Learn about the powerful tools and innovative techniques that can elevate your research. ???? #geneediting #CRISPR #biotech #scientificresearch

Read this publication from Nitulescu et al. to learn about non-viral gene editing via CRISPR-Cas12a resulting in up to 90% knock-in rates at the CD3ε locus in primary human T cells. ?? https://lnkd.in/e-z3aRQA ? #genomeediting #geneticengineering #CRISPR #lifescience

90% knock-in rates for non-viral gene editing of T cells! ?? One of the highest numbers I’ve seen so far! With the ongoing debate between viral vs. non-viral gene editing and the future of CAR-T cell therapies, it’s amazing to witness how far we’ve already come in ensuring the safety and efficacy of these life-saving treatments. The potential of non-viral methods is shaping up to be a game-changer for the industry. Exciting times ahead for gene editing and the future of immunotherapy! ???? #GeneEditing #NonViralTech #CARTCellTherapies #BiotechInnovation #FutureOfHealthcare #CellTherapy

Gene editing advancements are moving so fast the industry needs to be reminded of where these innovations are coming from. Controversy in the gene editing community has garnered the question, "Distinctions without a Difference: The Puzzling Argument About PASSIGE vs PASTE" @john finns Op-Ed from @Genetic Engineering & Biotechnology News dives into this apples-to-oranges comparison and begs the question “How will we turn the academic invention of large gene insertion into genomic medicines for patients in need?” Read about the facts here: https://lnkd.in/eHm_32Us

?? Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data Yuan and Duren presented LINGER, a machine-learning method to infer gene regulatory networks from single-cell paired gene expression and chromatin accessibility data. ? Key points ?? A fourfold to sevenfold relative increase in accuracy over existing methods ?? ?Incorporating atlas-scale external bulk data across diverse cellular contexts and prior knowledge of transcription factor motifs as a manifold regularization ?? Revealing a complex regulatory landscape of genome-wide association studies, enabling enhanced interpretation of disease-associated variants and genes ?? The estimation of transcription factor activity solely from bulk or single-cell gene expression data to identify driver regulators from case-control studies. ? More details: https://lnkd.in/eiytSjrv #clemsonuniversity #spatialomics #spatialbiology #singlecellanalysis #singlecell