Our March issue is online! https://lnkd.in/ey-6HdQr This themed issue coincides with the 50th anniversary of the Asilomar conference on recombinant DNA. We advocate for risk-appropriate, fact-based regulation of rDNA products. Reasonable people can disagree about the "right" lesson to learn from Asilomar. But our takeaway is this: the stringency of a preventive measure should match the assessed risk of the technology. Failure to use biotechnologies simply because they are biotechnologies is detrimental to sustainable human development. And when regulatory barriers become uncoupled from evidence-based risk, at best they are useless and at worst they undermine progress toward improved health, nutrition, and quality of life. Our fantastic cover image this month comes from Claire Williams at University of Saskatchewan. It combines a California vibe, images suggesting plant genetics, and a 70s palette that reminds us of our favorite vintage game shows. Special thanks to TIBTECH advisory board member Stuart Smyth for helping to organize the issue and collaborating on an editorial. Other articles in the collection include Risk-appropriate, science-based regulation Jose Falck-Zepeda Regulation of animal and plant agricultural biotech Simona Lubieniechi Alison Van Eenennaam Regulation in human health and genetic technology Hans-Georg Dederer Regulation of recombinant microbial products Trevor Charles And elsewhere in the issue: Neuroprivacy in human brain organoids Tsutomu Sawai Hiroshima University Phage-guided agents against bacterial infections Qi Wang Bin Li Plant genetic engineering with protein nanocarriers Temoor Ahmed Jason C. White Oxygen-generating scaffolds to enhance bone regeneration Warren Grayson Johns Hopkins Biomedical Engineering Cellobiose lipids Robert Pott Stellenbosch University C1 metabolism Jun Park UCLA Bioengineered yeast for preventing age-related diseases Codruta Ignea McGill University Synthetic microbial communities Rodrigo Ledesma-Amaro, PhD Imperial College London Biological upcycling of PET Tae Seok Moon J. Craig Venter Institute Waste stream valorization Neil Dixon The University of Manchester Microbial biomass production using oxic bioelectrosynthesis Johannes Gescher Hamburg University of Technology Reductive acetate uptake In-Geol Choi In Seop Chang Korea University Gwangju Institute of Science and Technology Engineered probiotic yeast Morten O. A. Sommer DTU Biosustain Quantification of cell-free DNA for prenatal genetics and oncology David Tsao BillionToOne
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Trends in Biotechnology, a Cell Press journal, publishes reviews and original research in the applied biological sciences: useful technology derived from or inspired by living systems. It addresses what is new, significant, and practicable in the integrated use of many biological technologies--from molecular genetics to biochemical engineering.
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Online now: Unlocking the potential of stem-cell-derived ‘synthetic’ embryo models https://dlvr.it/TJbqlB
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Shape the Future of Sustainable Agriculture at Cell Symposia: Towards Sustainable Agriculture October 19–21, 2025 | Sanya, China Join an exceptional lineup of Cell Press editors, including Yuli Ding (@Dev_Cell), Matthew J. Pavlovich (@TrendsinBiotech), and Yang Yang (@CellCellPress), at this groundbreaking symposium. https://hubs.li/Q03c3D3f0
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Shape the Future of Sustainable Agriculture at Cell Symposia: Towards Sustainable Agriculture October 19–21, 2025 | Sanya, China Join an exceptional lineup of Cell Press editors, including Yuli Ding (@Dev_Cell), Matthew J. Pavlovich (@TrendsinBiotech), and Yang Yang (@CellCellPress), at this groundbreaking symposium. https://hubs.ly/Q03c3MD90
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Online now: From academia to real-life biotech: ‘It takes a village’ https://dlvr.it/TJZfvY
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?? Engineering microbes for CO2 valorisation just got easier ?? ? The CO2-eating bacterium Cupriavidus necator is primed for application in sustainable biotechnology, yet was in dire need of tailored methods for genetic manipulation. Our collaborative effort on developing an efficient tool for genome engineering in this microbe is finally in press over at Trends in Biotechnology (link to paper: https://lnkd.in/dP7ZZY6C ). For a summary of the technology that we developed, check out my brilliant co-author Enrico Orsi’s post. A few “spoilers”: ?? Both Cas9 and Cas12a-mediated genome editing in C. necator. ? A new and improved electroporation protocol for this bacterium. ? Accelerated mutant generation – from standardised plasmid construction to deletion mutants in less than a week. ?? The development of SIBR2.0, or how to control gene expression by splitting a gene in two! ?? Custom sequence design software implemented in Python to help lower the barrier of entry for new users of this technology. This story was part of my PhD work with Wei E. Huang and Harrison Steel at the University of Oxford, Department of Engineering Science. When I started my project, I had fairly ambitious metabolic engineering goals for C. necator, but quickly realised that my efforts would be limited by the difficulty of both delivering plasmids and generating deletion mutants. Stubborn as I am, I decided to tackle this challenge head on, taking advantage of Constantinos Patinios’ then newly-minted SIBR technology. I am delighted that our technology is now available to all researchers in the field (and beyond), accelerating the application of non-model microbes in sustainable bioproduction. This wouldn’t have been possible without a small army of collaborators. Huge thanks to Luc Jansen, Evangelia-Niki Pentari, Sjoerd Creutzburg, Chase Beisel, Pablo Ivan Nikel , Raymond Staals, Nico Claassens, and John van der Oost. In particular, thank you to Enrico Orsi and Constantinos Patinios for their exceptional effort and leadership on this project, which we worked on tirelessly as we moved across jobs, countries and even continents. Their dedication, openness, and transparency made it an incredibly rewarding process. Do not hesitate to get in touch if you are interested in using this technology or collaborating on related projects!?
?? Advancing CRISPR tools for a greener (and CO2 neutral) future ?? #Microorganisms have the potential to capture and convert #CO? into valuable #molecules, paving the way for a more sustainable future. However, to fully harness their power, we need advanced #genome #editing #tools that allow us to program them efficiently. ?? That’s why I’m excited to share our latest work where we developed an improved #CRISPR/Cas system adapted for Cupriavidus necator—a promising microbial platform for CO?-based biomanufacturing. ?? When I first started working with C. necator in 2020, gene deletions were a major challenge. Now, more than four years later, we have optimized a Self-splicing Intron-Based Riboswitch (#SIBR) system to regulate Cas expression at the translational level. By strategically pausing Cas counter-selection, even the slowest homologous recombination processes can create mutants efficiently. ?? ?? Key highlights: ? Works with both Cas9 and Cas12a (SIBR2.0) ? High efficiency (70%+ mutation success) ? Faster turnover—mutant generation time halved compared to previous methods (about 4 days) This achievement wouldn’t have been possible without an incredible international team who put a lot of effort on it. Throughout this (long) journey, Simona Della Valle, Constantinos Patinios, and I moved across countries while developing this system. Simona pioneered the use of Cas12a in C. necator (making of it the great highlight of her PhD!), while myself, with the support of Luc Jansen and Evangelia-Niki Pentari, focused on optimizing the use of Cas9. Costas brought his expertise with SIBR, guiding its seamless integration. A huge thank you to our co-authors Sjoerd, Chase Beisel, Harrison Steel, Pablo Ivan Nikel, Raymond, Nico Claassens, John van der Oost, and Wei for their valuable contributions and support in making this possible! ?? Link to the paper: https://lnkd.in/dP7ZZY6C ?? What’s next? The plasmids will be available on Addgene, and we’re eager to see how this technology will drive new innovations in biotechnological CO? capture and conversion! If you're interested in using this system, feel free to reach out. Let’s push the boundaries of synthetic biology for a greener future! ???? #SyntheticBiology #CRISPR #CO2Capture #MetabolicEngineering #GreenBiotech
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Online now: Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system https://dlvr.it/TJYhBv
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Online now: Light-induced programmable solid–liquid phase transition of biomolecular condensates for improved biosynthesis https://dlvr.it/TJXyTZ
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Online now: OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window https://dlvr.it/TJX2X5
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Excited to see this work out at Trends in Biotechnology :) This is the first study I have coordinated from start to finish, and it brings me immense satisfaction to see it published. For a brilliant description of the technology we developed and its potential impact, Enrico Orsi explains it below. Thanks to all co-authors Simona Della Valle, Enrico Orsi, Sjoerd Creutzburg, Luc Jansen, Evangelia-Niki Pentari, Chase Beisel, @Harrison Steel, Pablo Ivan Nikel, @Raymond Staals, Nico Claassens, John van der Oost, and @Wei Huang. Lastly, I would like to say a huge thank you to the amazing Simona Della Valle and Enrico Orsi, for pushing this project forward, for never quiting and for believing in the tech and the team. You are the best!
?? Advancing CRISPR tools for a greener (and CO2 neutral) future ?? #Microorganisms have the potential to capture and convert #CO? into valuable #molecules, paving the way for a more sustainable future. However, to fully harness their power, we need advanced #genome #editing #tools that allow us to program them efficiently. ?? That’s why I’m excited to share our latest work where we developed an improved #CRISPR/Cas system adapted for Cupriavidus necator—a promising microbial platform for CO?-based biomanufacturing. ?? When I first started working with C. necator in 2020, gene deletions were a major challenge. Now, more than four years later, we have optimized a Self-splicing Intron-Based Riboswitch (#SIBR) system to regulate Cas expression at the translational level. By strategically pausing Cas counter-selection, even the slowest homologous recombination processes can create mutants efficiently. ?? ?? Key highlights: ? Works with both Cas9 and Cas12a (SIBR2.0) ? High efficiency (70%+ mutation success) ? Faster turnover—mutant generation time halved compared to previous methods (about 4 days) This achievement wouldn’t have been possible without an incredible international team who put a lot of effort on it. Throughout this (long) journey, Simona Della Valle, Constantinos Patinios, and I moved across countries while developing this system. Simona pioneered the use of Cas12a in C. necator (making of it the great highlight of her PhD!), while myself, with the support of Luc Jansen and Evangelia-Niki Pentari, focused on optimizing the use of Cas9. Costas brought his expertise with SIBR, guiding its seamless integration. A huge thank you to our co-authors Sjoerd, Chase Beisel, Harrison Steel, Pablo Ivan Nikel, Raymond, Nico Claassens, John van der Oost, and Wei for their valuable contributions and support in making this possible! ?? Link to the paper: https://lnkd.in/dP7ZZY6C ?? What’s next? The plasmids will be available on Addgene, and we’re eager to see how this technology will drive new innovations in biotechnological CO? capture and conversion! If you're interested in using this system, feel free to reach out. Let’s push the boundaries of synthetic biology for a greener future! ???? #SyntheticBiology #CRISPR #CO2Capture #MetabolicEngineering #GreenBiotech
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