Pfeiffer Genotyping

Advancements in Rubus idaeus (red raspberry) genomics! Building upon the foundational work of Clare et al. (2023 https://lnkd.in/gUdSAhEQ), our team has developed and validated 48 PACE markers. These markers were selected based on concordance between a multispecies Axiom array and whole-genome sequencing data from 210 red raspberry accessions. In addition, we've expanded our molecular toolkit with the development and validation of SNP panels tailored for private breeding programs: Red Raspberry: 24 SNPs derived from a multispecies Axiom array. Blackberry: 72 SNPs based on a multispecies Axiom array. These robust Rubus SNP panels are instrumental for advancing breeding programs, offering applications in: Assessing population diversity Conducting parentage analysis Resolving incorrect pedigrees Fingerprinting and clonal identification Intellectual property protection By integrating these SNP markers, breeders can streamline breeding processes, and expedite the development of superior Rubus cultivars. Contact: [email protected]

Exciting news for blackberry breeders! ???? Diagnostic PACE marker is now available for the prickle-free trait in tetraploid blackberries. Developed using the latest research from Margaret Worthington's group (Dec 2024), identified the prickle-free locus in an extensive LD block at the distal end of chromosome 4: 30.48 Mb–36.04 Mb, with the peak SNP at 33,636,565 bp. The PACE assay for the thornless trait boasts a prediction accuracy of >95%, making it a powerful tool for marker-assisted selection. The marker is now offered as part of the marker-assisted selection services at Pfeiffer Genotyping. For inquiries, reach out to Navneet Kaur at [email protected]

Exciting news for blackberry breeders! ???? Diagnostic PACE marker is now available for the prickle-free trait in tetraploid blackberries. Developed using the latest research from Margaret Worthington's group (Dec 2024), this breakthrough identified the prickle-free locus in a high-LD block at the distal end of chromosome 4: 30.48 Mb–36.04 Mb, with the peak SNP at 33,636,565 bp. The PACE assay for thornless trait boasts a prediction accuracy of >95%, making it a powerful tool for marker-assisted selection. The marker is now offered as part of the marker-assisted selection services at Pfeiffer Genotyping. For inquiries, contact at [email protected].

Exciting Development in Blackberry Genotyping! Pfeiffer Genotyping is thrilled to announce the launch of our cutting-edge SNP panel tailored for autotetraploid blackberries (2n=4x=28), based on the groundbreaking work published by Sara M. et al. in G3 in October 2023. Their innovative study, "A multiplexed plant–animal SNP array for selective breeding and species conservation applications," laid the groundwork for this advanced tool. Key Highlights: Comprehensive Dataset: Our panel is developed from a WGS dataset of 27 blackberry accessions, featuring 1,864 SNPs distributed across the genome, and 2,299 SNPs in genes associated with key traits such as sweetness, thornless, and flowering. Mini Panel Precision: We’ve meticulously selected a mini panel of 24 SNPs, converted from axiom probes to PACE markers, focusing on unique hits, positioning away from centromeres and telomeres, and ensuring even genome coverage. Versatile Applications: This panel is designed for genetic diversity studies and fingerprinting, offering valuable insights for breeding. For more details or to get started with this innovative genotyping tool, feel free to reach out at [email protected]