Olaris, Inc

Our #metaboliteofthemonth N-acetylneuraminic acid (Neu5Ac) is part of the kidney’s glomerular filtration barrier. This sialic acid, produced in the hexosamine biosynthetic pathway, attaches to glycans on the surface of glomerular cells. Glycoproteins and glycolipids sialylated with Neu5Ac form part of a the glycolyx, a negatively charged, gel-like layer that acts like a sieve, preventing proteins from leaking out of the blood into urine. Sources: Fuentes, F et al. (2020) Kidney360.1(9):957. doi: 10.34067/kid.0002122020; Ito M (2012) PLoS ONE 7(1): e29873. https://lnkd.in/ezv2F_k7; Pawluczyk, IZA et al. (2015) Laboratory Investigation. 95(9),1019. https://lnkd.in/eC-rxbji #MetaboliteOfTheMonth #Neu5Ac #SialicAcid #KidneyHealth #GlomerularFiltration #Glycans #Glycocalyx #HexosamineBiosynthesis #KidneyFunction #RenalResearch #Kidney360

Managing diabetes can protect the health of kidneys. Diabetes is the leading cause of kidney disease. Managing sugar levels and blood pressure can decrease damage to blood vessels in the kidneys. Source: National Institute of Diabetes and Digestive and Kidney Diseases: Diabetic Kidney Disease (https://lnkd.in/eE_SaCpv) #DiabetesManagement #KidneyHealth #DiabeticKidneyDisease #BloodSugarControl #BloodPressureManagement #PreventKidneyDisease #HealthyLiving #DiabetesCare #KidneyProtection

#TBT In the early days of Olaris our incredible friends and families generously donated urine samples to help us develop and optimize the myOLARIS diagnostic platform! THANK YOU!! Your contributions played a vital role in advancing our precision diagnostic platform and bringing us one step closer to transforming how we diagnose and treat diseases. Science takes a village, and we’re beyond grateful for your support. #TBT #ThankYou #ScienceCommunity #PrecisionDiagnostics #HealthInnovation #myOLARIS #MedicalResearch #Gratitude #SupportScience #HealthTech #BreakthroughsInScience

At our latest journal club, Dr. Masoumeh Dorrani reviewed a study published in the Journal of Chromatography B. The research developed and successfully validated a sensitive, specific, and fast U-HPLC–MS/MS assay for quantifying MPA and its major metabolites in plasma and urine. This assay includes automated sample preparation using a robotic system with 96-well plates. The study highlights the advantages of LC/MS assays in measuring immunosuppressants due to their high specificity. Key achievements include the complete separation of MPA, MPAG, and AcMPAG, as well as relatively short analytical run times of just 2.3 minutes between injections. Unlike previously published assays, this method overcomes the limitations of extensive multi-step sample preparation and includes the analysis of important metabolites like AcMPAG. #PharmaceuticalResearch #LCMS #UHPPLC #Immunosuppressants #MetaboliteAnalysis #AnalyticalChemistry #MassSpectrometry #Pharmacokinetics #JournalClub #BiotechResearch

Congratulations to CEO Liz O'Day who has been nominated as a candidate for Medical Sciences Trustee for United States Pharmacopeia (USP). USP was founded in 1820 by physicians who wanted to protect patients from poor-quality medical prepartions. It has grown into a nonprofit body of 300 delegates representing state and national associations and colleges of medicine, nursing, pharmacy, industry and agencies of the federal government. USP sets quality standards for medicines, food ingredients, and dietary supplements to ensure they are safe, effective, and properly manufactured. These standards are used across the globe to maintain consistent quality across pharmaceuticals and other health-related products. Congratulations Liz on your well-deserved nomination! ??? #Leadership #USP #MedicalSciences #Pharmaceuticals #QualityStandards #HealthcareExcellence #Innovation #HealthcareLeadership #WellDeserved

#MetaboliteoftheMonth alert! Say hello to N-acetylneuraminic acid (Neu5Ac) -- the only sialic acid synthesized de novo in humans. Sialic acid-containing structures on cell surfaces have important physiological functions, like keeping blood flowing smoothly. Sialic acids are negatively charged nine-carbon monosaccharides. Neu5Ac is synthesized in the hexosamine pathway and converted to CMP-Neu5Ac in the nucleus. Then in the Golgi, sialic acid modifies the ends of glycoproteins and glycolipids destined for the cell surface. Charge repulsion between sialylated red blood cell surfaces and sialyated blood vessel surfaces (endothelial glycocalyx) prevents red blood cells from sticking. Source: Zhu, W et al.(2024) Cell Death Discov. 10, 415. https://buff.ly/xihui6N #MetaboliteOfTheMonth #Neu5Ac #SialicAcid #HexosaminePathway #CMPNeu5Ac #Glycosylation #BloodFlow #EndothelialGlycocalyx #Sialylation #Glycobiology #CellSurfaceModification

Kidneys help regulate osmolarity, which is the concentration of dissolved particles in body fluids. Maintaining stable osmolarity in body fluids keeps cells healthy. Kidneys maintain osmolarity by adjusting the amount of fluids and electrolytes excreted from the body. Kidneys #Osmolarity #BodyFluids #Homeostasis #RenalFunction #WaterBalance #KidneyHealth

It's CEoT. The annual "Cutting Edge of Transplantation (CEoT)" kicks off in AZ today. This year's theme, "Transforming Tomorrow: Leading the Way in Organ Transplantation Modernization," invites transplant professionals to explore how artificial intelligence, alongside other innovations, strategies, and challenges, is shaping the future of the field. We can't wait to learn more! #CEoT2025#AIInHealthcare#TransplantInnovation#TransformingTomorrow#FutureOfTransplantation

?? MS-Based Sample Prep in Metabolomics ?? Several studies have refined urine & plasma sample prep for MS-based metabolomics, helping improve reproducibility and efficiency. Key methods include: ? Solid-phase extraction (SPE) & solvent precipitation for cleaner samples ? Incubation, sonication, & mild centrifugation to enhance extraction ? Dilute-and-shoot strategy for high-throughput workflows These insights will help optimize Olaris' MS-based metabolomics protocol! ?? ?? Question for authors: How does mild centrifugation or filtration affect metabolite retention in urine samples? Olaris Journal Club, Nihel Bekhti #Metabolomics #MSAnalysis #SamplePreparation #MetaboliteScience #Olaris