O2M Technologies

If you don't see it, you don't know. Here are oxygen maps of two islet cell-seeded devices for curing T1D. Do you know which one has live cells?

Do you know that the body controls oxygen concentration tightly? Here is a snapshot of our upcoming article showing the mouse body pO2 as a function of breathing rate. Stay tuned for our upcoming article, and check out our website (https://oxygenimaging.com/) for more information on oxygen imaging.

EPROI as a ground truth to correct PET hypoxia threshold! https://zc.vg/DFWJw?m=0 World Molecular Imaging Society Inna Gertsenshteyn INTERNATIONAL SOCIETY FOR MAGNETIC RESONANCE IN MEDICINE

If you don't see it, you don't know. Here are oxygen maps of two islet cell-seeded devices for curing T1D. Do you know which one has live cells?

EPROI as a ground truth to correct PET hypoxia threshold! https://zc.vg/DFWJw?m=0 World Molecular Imaging Society Inna Gertsenshteyn INTERNATIONAL SOCIETY FOR MAGNETIC RESONANCE IN MEDICINE

Do you know that the body controls oxygen concentration tightly? Here is a snapshot of our upcoming article showing the mouse body pO2 as a function of breathing rate. Stay tuned for our upcoming article, and check out our website (https://oxygenimaging.com/) for more information on oxygen imaging.

Cell viability is an important measurement in all biological labs. Currently, it is performed using assay-based methods?that, ironically, kill the very cells that are being checked as live cells. In our recent work (published in #npj imaging), we show that we can utilize the power of nondestructive EPR oxygen imaging for 3D and longitudinal viability assessment. More importantly, it shows that the cells with different seeding densities have different pO2, even in the incubator (are you subjecting your cells to hypoxia unknowingly?). We show that we can assess cells' metabolic activities in biomaterials (how many cells get lost during cell seeding?). This is an important assay for every lab?that works with cells, as different oxygen concentrations can lead to vastly different outcomes. Our instrument, JIVA-25, was utilized for all experiments. Tim Witney Carl Simon Greta Babakhanova Safa Hameed Navin Viswakarma Henry Ahn Check out the full paper here: https://lnkd.in/gRZXx58E

Delving further on using oxygen imaging for cell viability (https://lnkd.in/g95WG-7G): A few key takeaways: 1. We can assess cells’ metabolic activities longitudinally and in 3D without destroying them in the process. 2. We can distinguish between live and dead cells. 3. The cells can be hypoxic even in the incubator. 4. We can assess cells’ metabolic activity in a?culture medium, but more importantly, when also seeded in a hydrogel. 5. We measure the?oxygen consumption rate in equilibrium, which is different from the current method of measuring OCR by starving cells. 6. The oxygen concentration of wells with low cell density is high and decreases exponentially with increasing cell seeding density. However, it changes with time as cells become dormant, differentiate, propagate, or die. Therefore, cell density is an important?factor to control when using them for cell therapy and tissue engineering. This is an important assay for every lab?that works with cells, as different oxygen concentrations can lead to vastly different outcomes. Could this be a factor contributing to the reproducibility crises in biological sciences?

Many acute (ischemic and hemorrhagic stroke, traumatic brain injury, cerebral malaria, and other central and systemic infections) and chronic (Alzheimer's disease, cerebral small vessel disease, and multiple sclerosis) neurological disorders are associated with the compromise in the blood-brain barrier and altered brain metabolism, and currently, there is no good way to image and quantify it. We present a simple and straightforward method to image BBB leakage and brain pO2 using our key technology, trityl-based eMRI and preclinical instrument, JIVA-25, in preclinical models. Check out the complete study: https://lnkd.in/dRaNETqc

Join us for O2M's presentations if you are in Montreal for WMIC 2024! World Molecular Imaging Society European Society for Molecular Imaging - ESMI Euro-BioImaging