Multi-Omics AWG - NASA GeneLab转发了
B.Pharm, Ph.D., Associate Professor, Senior Researcher - Lisbon Medical School, University of Lisbon.
Human long interspersed nuclear element-1 (LINE-1 or L1) is an autonomous retrotransposon that comprises approximately 17% of the human genome. LINE-1 elements are capable of self-propagation through an RNA-mediated process called retrotransposition, which contributes to genetic variation and genome evolution. The open reading frame 2 protein (ORF2p) is a crucial component of the LINE-1 retrotransposition machinery, facilitating enzymatic activities essential for integration into the genome. LINE-1 elements are the only active autonomous transposable elements in the human genome. They consist of two open reading frames, ORF1 and ORF2, flanked by untranslated regions (UTRs). ORF1p encodes an RNA-binding protein with nucleic acid chaperone activity, while ORF2p harbors enzymatic domains essential for retrotransposition. ORF2p plays a pivotal role in the mobilization of LINE-1 elements by mediating the key biochemical steps necessary for their insertion into new genomic loci. While LINE-1 retrotransposition is a source of genetic diversity, unchecked ORF2p activity can have deleterious effects on genome integrity. ORF2p is an essential protein for LINE-1 retrotransposition, possessing enzymatic activities that facilitate the integration of new genetic elements into the human genome. While it contributes to genetic diversity and evolution, its uncontrolled activity poses risks to genomic integrity and is linked to several diseases. Understanding the regulation of ORF2p and its interactions with cellular defense mechanisms may provide insights into novel therapeutic strategies to mitigate its deleterious effects. Here you can see a cryoEM structure of the human LINE-1 ORF2p target-primed reverse transcription complex (PDB code: 9HDO) #molecularart #transposon #line #reverse #transcriptase #cryoem Structure rendered with 3D Protein Imaging, post-processed with Dzine AI and depicted with @corelphotopaint