Integrated Protein Technologies, Inc.

?????? ?????????????????????? ??????????! The SampleStream Platform and Orbitrap-based CDMS combine to tackle AAV Content Ratio Analysis In this work, Dr. Sam Janisse and co-worked demonstrated the powerful coupling of the SampleStream Platform with Orbitrap-based charge detection mass spectrometry (CDMS) for the analysis of AAV content ratios. ?????? ???????????????????? ???? ?????????? ????????????????: ? Concentrating capability of SampleStream enables high volume injections (100 μL or higher) of low titer samples. ? Efficient buffer exchange enables analysis from culture media. Combined, these advantages provide a route to analyzing in-process AAV samples. ???????? ???????? ????????: https://lnkd.in/g8S4hG9D

Congrats to Ben Niu and his crew at BMS on this end-to-end pipeline for bi- and multispecific screening. For the SampleStream platform component of this broader workflow, two key things were illustrated. Perhaps the more important of the two, is that co-elution of half antibodies along with full antibodies does not suffer from significant bias due to different ionization efficiencies, except when the half antibody makes up >50% of the sample (not a good clone anyway). A total of 192 samples from multiple platforms all demonstrated this same behavior. The second important aspect is running at 1 min/sample. While there are other platforms capable of this level of throughput, the removal of a chromatographic resin, and the non-specific interactions that come along with it, make the SampleStream platform less sensitive to variations in properties such as hydrophobicity or pI. If you have a bi-/multispecific platform and aren't using the SampleStream platform for your screening already, please reach out to start a conversation. https://lnkd.in/gsAycm-4

Take a look at this manuscript by Benjamin Des Soye and colleagues at Northwestern University. Their research heavily leveraged the Plugin System from CTC Analytics AG, a tool that empowers programmers to teach the PAL 3 System how to control virtually any hardware with a digital communication interface. This allows the robot to seamlessly integrate with external hardware without the need to modify the drivers that link it to MS data acquisition software. Essentially, the additional hardware appears as native modules within the acquisition software. For instance, the SampleStream modules are entirely dependent on this system. In their work, the authors employed a plugin for the KingFisher, an automation platform designed for affinity purification, enabling immunoprecipitations (IPs) to be completed before processing samples via the SampleStream Platform—all within individual rows in the run list. IPT has also developed plugins for other devices, such as 2D barcode readers and heater/shakers. The flexibility of the plugin system gives IPT the ability to bring more hardware under automated control, creating comprehensive automation solutions. What additional functionality would you like to see integrated into the SampleStream Platform? https://lnkd.in/gE9_GGVa

Here is a quick little "capability note". A sample of NISTmAb with a 3.5x excess of BSA was processed in a flow cell with a 10 kDa MWCO membrane then transferred to a flow cell with a 3 kDa MWCO membrane for reduction. The BSA was completely removed. Sacrificial proteins are sometimes used as "blocking" materials to improve recovery from vials. Additionally, excess Fc has been used for native elution from affinity capture. Subsequent processing of this sample type on the SampleStream Platform can easily remove these high abundance species prior to analysis.

Take a look at our latest application note on in-channel reduction! By concentrating and buffer exchanging the sample before adding a reducing agent and chaotrope, you can achieve fast and consistent disulfide reduction. This method allows for multiple sample preps from a single vial, enabling more analyses with less sample volume and keeping you and your team off the bench.

Rachel Shi, PhD from Genentech, joins us for our next webinar: “Characterizing Antibody-Drug Conjugates with Charge Detection Mass Spectrometry,” on Wednesday, July 31st at 10 AM PT. Visit the link to read the speaker abstract and register to attend! https://lnkd.in/gA7zW8nj

Rachel Liuqing Shi delivering a great talk on high DAR ADC analysis using the SampleStream Platform as a key part of the workflow.

If you are attending ASMS 2024, be sure to catch Dr. Rachel Liuqing Shi giving a talk on DAR analysis of complex ADCs in biotransformation samples using CDMS automated with the SampleStream Platform.

If you are attending ASMS, be sure to swing past Dr. Sam Janisse's poster on automated AAV content analysis using the SampleStream Platform!