Well said partner.
?? Indee Labs posted a manuscript that applies TIMING? from CellChorus to show that CAR-T cells manufactured with the Indee platform have superior motility and resistance to activation-induced cell death (AICD). ?? Background: Indee is developing Hydropore, a non-viral technology that utilizes microfluidic vortex shedding to deliver small molecules, nucleic acids, proteins, and gene-editing complexes to immune cells with low impact to cell viability and function. The technology is designed to bypass the challenges and limitations of viral transduction and traditional transfection via electroporation, allowing scientists to accelerate their research goals from discovery to manufacturing. ???The approach: The team compared hydroporation with nucleofection?in the generation of CAR-T cells. ???Key findings from the research include: - Scaled-up hydroporation can process 5 x 10^8 cells in less than 10 seconds. - Hydroporation yields a higher number of transfected cells compared to both nucleofection and electroporation, partly because CAR-T made with hydroporation (Hy-CAR-T) robustly grew and divided. -?Hy-CAR-T cells CAR-T from nucleofection (Nuc-CAR-T) in terms of T cell subsets, activation, and pro-inflammatory cytokine secretion. - Hy-CAR-T secreted higher levels of the anti-inflammatory cytokine IL-10, which is linked to better CAR-T function. -?TIMING data showed that individual Hy-CAR-T target seeking. contact dynamics, and target killing were just as robust as Nuc-CAR-T. - TIMING measurements of cell movements during target engagement (synapse) showed that Hy-CAR-Ts maintained significantly more motility than donor-matched Nuc-CAR-T cells. Motility has been linked to strong CAR-T potency and patient response in other studies. - TIMING data showed that Hy-CAR-T were resistant to activation-induced cell death (AICD) compared with Nuc-CAR-T.?AICD contributes to exhaustion of engineered CAR-T cells ?? Read more: https://lnkd.in/g2S7JF85 Authors: Brandon Sytsma, Vincent Allain, Struan Bourke, Fairuz Faizee, Mohsen Fathi, Rebecca Berdeaux, Leonardo Ferreira, Jared Brewer, Lian Li, Fong Pan, Allison Rothrock, William Nyberg, Zongmei Lei, Leah Wilson, Justin Eyquem, ryan pawell Affiliations: University of California, San Francisco, Université Paris Cité médecine, Medical University of South Carolina, MUSC Hollings Cancer Center, Duke University School of Medicine, Institute for Human Genetics at UCSF, Parker Institute for Cancer Immunotherapy, Indee Labs, CellChorus #singleCell #CarT #cellTherapy #immunooncology #immunotherapy #ai #ml Y Combinator, BioTools Innovator