Columbus Instruments: Behavior, Physiology, & Metabolism

??? Your Muscles Might Have a Built-In Clock—And It Affects Your Metabolism! Researchers found that circadian clock regulators PER2 and RORα play distinct but interconnected roles in skeletal muscle function and metabolism. Deleting PER2 (primary clock loop) or RORα (secondary clock loop) in skeletal muscle disrupted metabolic homeostasis, glucose metabolism, and muscle function. Both knockouts reduced the amplitude of core clock gene expression but preserved circadian oscillations, affecting amino acid metabolism, mitochondrial biogenesis, and the tricarboxylic acid (TCA) cycle. PER2 deletion led to time-specific metabolic changes, reducing glucose tolerance and voluntary nighttime activity, while RORα deletion caused circadian-independent metabolic and mitochondrial alterations, improving exercise capacity and energy expenditure. Proteomic and metabolomic analyses confirmed widespread disruptions in amino acid metabolism and muscle plasticity, reinforcing the central role of the molecular clock in skeletal muscle regulation. These findings suggest that targeting circadian components could be a novel strategy to optimize muscle function and treat metabolic disorders. - In this study, our CLAMS System was used for evaluating food consumption, oxygen consumption, and CO2 production. CLAMs is a metabolic cage system for precise lab monitoring and easy data analysis. It is the comprehensive solution for metabolic and behavioral research. Learn more about our innovative ?????????????????? ???????? ????????????:https://lnkd.in/gkpGANMm

Columbus Instruments is headed to the Keystone Symposia on Obesity and Adipose Tissue in Banff, Canada, from February 23-26, 2025! We’re thrilled to exhibit and showcase CLAMS, a powerful tool for tracking and analyzing metabolic activity. With its precision and advanced capabilities, CLAMS enables researchers to gain deeper insights into metabolic processes and energy expenditure, helping accelerate breakthroughs in health and disease research. ?? ?? Be sure to stop by our booth to see the CLAMS system and learn how it can enhance your research. We’re excited to connect with fellow scientists and innovators! #KeystoneSymposia #MetabolismResearch

The use of medications like Wegovy, Ozempic, and Zepbound has skyrocketed as people turn to GLP-1 medications to treat diabetes and obesity. While these medications are mainly associated with improved insulin sensitivity and weight loss, researchers are learning that the benefits of these GLP-1 drugs may go well beyond these effects. In addition to research on how GLP-1 drugs may help with addiction and lower risk for certain cancers, a new review shines a light on how this class of medications may provide some degree of brain health protection. The review examines how GLP-1 drugs can balance the neurovascular unit — the part of the brain that regulates blood flow within the brain — thereby creating the possibility of improving cognitive functioning. ??Read more: https://lnkd.in/emq6P_7j - Looking for a comprehensive solution for your metabolic research? Columbus Instruments stands as the industry leader in instrumentation for monitoring metabolism, behavior, and physiology. Learn more about our comprehensive lab monitoring system (Oxymax-CLAMS) - an automated, high-precision, customizable & configurable solution for advanced laboratory research. https://lnkd.in/gkpGANMm

??Top Posts Throwback? Warm-sensitive neurons (WSN) within the #preoptic area (POA) of the #hypothalamus were thought to sense internal warmth, using this information to regulate #bodytemperature. However, the function of WSN in mice remains unknown. So, Zhou and colleagues aimed to understand better the cellular and molecular mechanisms by which WSNs measure temperature. Methods? The researchers designed an implantable device for heating the brain in freely behaving mice. They also conducted genetic manipulation and pharmacological studies to explore the molecular mechanisms of WSNs. The Comprehensive Lab Animal System (#CLAMS, #ColumbusInstruments) was used to monitor energy expenditure, with data collected at 10-minute intervals. Mice were acclimated to the chambers for two days before receiving saline and Englerin A during the dark phase. Results? The main findings of these experiments were:? - When the TRPC4 channel was silenced in mice, the hypothermia caused by light-mediated heating of the POA was significantly reduced.? - Losso of function studies confirmed the role of TRPC4 in warm sensing in GABAergic WSNs. This disruption led to defects in temperature regulation, warm defense, and fever response.? - TRPC4 antagonists and agonists are involved in regulating body core temperature. Conclusion? TRPC4 is essential for warmth sensing and may represent a potential therapeutic target for managing body core temperature. Would you like to run a similar experiment in your lab? Learn more about our products on our website: https://colinst.com/ CLAMS - Comprehensive Lab Animal Monitoring System: https://lnkd.in/gkpGANMm More details of the study: https://lnkd.in/gkRWmEku

Intracerebral hemorrhage (ICH) is a severe type of stroke. Persistent hyperglycemia following #ICH is linked to worsened brain function and death. However, the worsening effect of #hyperglicemia on ICH injury at the molecular level is still unclear. A recent study by Alhadidi and colleagues looked at the impact of #diabetes on ICH injury using a non-obese diabetic (NOD) mouse model of type I diabetes. Check the infographic below for more info! Would you like to run similar experiments in your lab? Visit https://www.colinst.com/ to learn more. Rotamex-5 Rotarod: https://lnkd.in/dUxA_Xsr Grip Strength Meter: https://lnkd.in/gpK2T6sy

Opsin3 is a protein that helps regulate adipocyte function. However, the mechanisms by which #Opsin3 affects #metabolism in response to long-term light exposure are not clear. A new study by Tsuji and colleagues found that blue light could reduce lipid accumulation in subcutaneous white adipose tissue (#WAT), improving #obesity-induced metabolic abnormalities. Check the infographic for more! Would you like to run similar experiments in your lab? Visit https://www.colinst.com/?to learn more. CLAMS - Comprehensive Lab Animal Monitoring System: https://lnkd.in/g5mkwxi3

?? Are you finding a comprehensive solution for your rodent metabolic research? Introducing our Comprehensive Lab Animal Monitoring System (Oxymax-CLAMS) - an automated, high precision, customizable & configurable solution for advanced laboratory research. Oxymax-CLAMS allows for 24-hour, automated, non-invasive collection of gas exchange - and therefore Total Energy Expenditure - in the context of behavioural parameters like locomotion, wheel running, and sleep. Swipe the carousel to learn more! For more information, please see: https://lnkd.in/gkpGANMm Columbus Instruments stands as the industry leader in instrumentation for monitoring metabolism, behaviour, and physiology in lab animals.?

??Hindbrain REV-ERB nuclear receptors regulate sensitivity to diet-induced obesity and brown adipose tissue pathophysiology Obesity is a major public health issue with myriad treatment options there is a lack of highly effective, long-term therapies. Although development of obesity is complex and multifactorial, obesity can be characterised as a disease of energy balance dysregulation in the brain. A recent study reveals how hindbrain REV-ERBα/β nuclear receptors influence sensitivity to diet-induced obesity (DIO) and brown adipose tissue (BAT) function. These receptors, critical for metabolic regulation, were studied in the dorsal vagal complex (DVC), a hub for integrating metabolic signals. By creating a double knockout (DVC RDKO) of REV-ERBα/β in mice, researchers uncovered striking metabolic disruptions under high-fat diets. The DVC RDKO mice showed increased weight gain, reduced metabolic rates, and impaired BAT activity, with lower expression of BAT-related genes and diminished sympathetic outflow. The findings also linked these effects to reduced calcitonin receptor expression in the DVC, underscoring its role in energy balance and sympathetic regulation. This study highlights the central role of REV-ERBα/β in maintaining energy homeostasis, offering new insights into obesity’s underlying mechanisms and potential therapeutic targets. Read More: https://lnkd.in/enEAtTfg

Recent research underscores the significant role of the gut microbiome—the complex community of microorganisms residing in our digestive tract—in influencing how we manage stress. Published in Nature Mental Health, the study identified specific biological patterns in the gut microbiomes of individuals who exhibit high resilience to stress. These findings suggest that the gut-brain connection is pivotal in determining our stress responses. Dr. Arpana Church, a neuroscientist at UCLA’s Goodman-Luskin Microbiome Center and lead author of the study, remarked on the precision of these patterns, highlighting the potential for future research to develop targeted treatments. This study also opens avenues for identifying microbiome biomarkers that could inform personalized mental health therapies. Read More: https://lnkd.in/gjD68PCc - Conducting pre-clinical biology research? Columbus Instruments stands as the industry leader in instrumentation for monitoring metabolism, behaviour, and physiology in lab animals. With expertise spanning over 46 different applications in life sciences research, we are your go-to resource. Discover the perfect product for your research needs on our website: https://lnkd.in/eBQC2kCN

? #ThrowbackThursday ? Back to 2002! Here’s a look at the impressive craftsmanship behind the original "Fast Response Calorimetry Cages" ??—engineered to act as closely as possible to a “mouse-mask.” These pioneering cages became invaluable for studying the acute effects of various compounds on energy production and enabled precise measurements of VO? & VCO? in mouse pups. By 2003, Version 2.0 brought activity measurements into the mix. Even today, these innovative systems help bridge the gap between measurement techniques for masked-humans and caged-mice ??. ?? Curious about the latest tech? Discover our Oxymax-CLAMS-Connect, a cutting-edge metabolic phenotyping system that’s advancing the science of today and tomorrow! ?? Learn More Here: https://lnkd.in/gkpGANMm #MetabolicPhenotyping #EnergyProduction #ScientificInnovation #Research #LifeSciences #Throwback