Azure Biosystems

New #publication spotlight from PLOS Pathogens using fluorescent imaging from the Sapphire Biomolecular Imager to determine IC50 using in-cell Western. ?? While the emergency phase of the pandemic is considered to be over by the WHO, COVID-19 remains a circulating respiratory virus that continues to evolve and cause illness globally. In particular, SARS-CoV-2 continues to mutate, leading to new subvariants with varying degrees of transmissibility and immune evasion. In a PLOS Pathogens paper, Dong et al isolated several promising candidates for disrupting the cycle of immune selection and viral escape through effectively blocking respiratory acquisition and transmission of diverse sarbecoviruses. The Sapphire Biomolecular Imager was used to determine the neutralizing activity of the top nanobody candidates through fluorescence in-cell Western. Read the full Spotlight here: https://bit.ly/4kmwXnP

In drug discovery, accurately determining a compound's potency (IC50) is critical. While traditional methods can provide valuable information, In-cell Western assays offer several key advantages. Our latest blog post delves into these advantages in detail, explaining why In-cell Western is becoming the preferred method for many researchers. Read the blog post here -> https://bit.ly/4hhfaMQ

In case you missed it, we're exhibiting with MESLO at MedLab 2025 from 3-6 February! Be sure to stop by booth Z6.J03 to meet, Muzaffer Emre Gul, our Technical Product Specialist and check out the Cielo 6. #mymedlab #medlabmiddleeast #azurebiosystems

Fluorescent Western blotting has revolutionized the field of protein analysis by providing a highly sensitive and specific method for detecting and quantifying proteins in complex mixtures. But how do you get started? As with any technique, a protocol. Our Fluorescent Western blotting protocol is suitable for researchers of all levels and will guide you through each stage of the process, from sample preparation to image analysis. With this protocol, you will be able to confidently generate high-quality data for your research. https://bit.ly/42wWHHl #westernblot #azurebiosystems

Obtaining high-quality purified proteins is essential for structural biology experiments. Membrane protein purification is particularly complex, as evidenced by the fact that less than 1% of entries in the Protein Data Bank represent membrane proteins. A method put forth by Drew et al. (2008) involving SDS-PAGE has proven useful in the purification of fluorescently labeled membrane proteins. Our recent app note explores an adapted protocol, in which the Sapphire Biomolecular Imager provides optimized in-gel fluorescence detection for semi-quantifying and monitoring the purification of GFP-labeled membrane proteins from yeast. Read full app note here -> https://bit.ly/3ZmEY2h Figure 1: Detection of five different eGFP-tagged membrane proteins. A) and B) Fluorescence images of SDS-PAGE obtained with Azure Sapphire Biomolecular Imager. C) Integrated fluorescence density from different purified membrane proteins.

Our last day of #AMP2024 is tomorrow! Stop by booth #1601 for a chance to win a Sapphire FL Bimolecular Imager lego kit!

Azure Biosystems has arrived at #AMP2024! Come and stop by booth 1601 and say hi!

We’re thrilled to be at the AMP conference next week in Vancouver, connecting with so many brilliant minds in pathology and molecular diagnostics. If you're attending, be sure to stop by booth #1601 to explore our latest innovations in fluorescent imaging and qPCR. Come chat with our team, get a hands-on demo, and discover how we're advancing the way you image. See you at AMP! ?? #AMP2024

Last day at #SfN24! We’ve had a fantastic time connecting with the future of neuro. Still a couple more hours to stop by booth #541 to learn about how to get the most from your western blots ??

Ready for day 2 at #SfN24! If you stopped by our booth yesterday, check your email today. You could be a winner of a new LEGO set! We’re at booth #541 again today from 9:30-5pm ??