Alpenglow Biosciences

?????????????????????? ????????: ?? ????????-?????????????? ???? ???? ??????????????! ?? ?????????????????? ?????????????????????? is excited to unveil ???????? (??????????-?????????? ???????? ???????????????????? ??????????????????), a revolutionary interface designed to take your ?????????? (???????????? ????????-?????? ??????????-??????????) microscope to new heights! ?? Get ready to experience light-sheet microscopy like never before. Join us at ???????? ???????? for an exclusive first look at LUMI! ?? ???????? is crafted to integrate smart microscopy into the light-sheet workflow. Delivering unmatched efficiency and fast results making high-quality ???? ?????????????? easier and more accessible than ever. Here’s why you’ll love it: ?? ???????????????? ???????? ?????????? – Set up your scans in a flash with an intuitive interface that requires no advanced knowledge. ?? ???????? ???????????????? ???????? ?????????? ???????????? – ?????????? ???????? lets you see wide-field views in real-time, while ???????? ???????? delivers high-resolution imaging that captures every detail with precision. ?? ?????????? ???????????????????? – Ability to create algorithms for automated region of interest detection and Scout-to-Zoom workflows. “???????? ???? ???????????????????????? ???? ??????????????, ???????????? ???? ????????????, ??????????????, ?????? ???????? ???????????????????? ???? ?????????? ????????????????????,” says Nicholas Reder MD, MPH, CEO founder and co-inventor at Alpenglow Biosciences. ?? Don’t miss out! ?????????????? ?? ??:?? ?????????????? ???????? Steve Pemberton, SVP of Commercial Operations at Alpenglow Biosciences, at ABRF 2025 to explore how LUMI is shaping the future of spatial biology: https://lnkd.in/dzis5gj7 ?? Take a sneak peek with the short video below, drop your comments, and let us know what you think of LUMI! We’d love to hear your thoughts and appreciation. Read the full Press Release: https://lnkd.in/dGTqtBR9 #3DImaging #SpatialBiology #Microscopy #ABRF2025 #AlpenglowBiosciences

Happy #FluorescenceFriday! Ready to be wowed? Today, we’re diving into the fascinating world of prostate organoids! ?? These 3D structures deserve a 3D approach—and that’s where LUMI steps in. ?? With LUMI, you can now seamlessly image large areas at low resolution and then zoom in on regions of interest at ultra-high resolution using our incorporated Smart Microscopy features! It’s never been easier to analyze complex organoid structures with pinpoint accuracy. ?? Learn more in our recent PR release! https://lnkd.in/dGTqtBR9 The prostate organoids are stained with TO-PRO-3 and Eosin, then pseudocolored to reveal every detail. ?? Join the revolution. ?? Dive deep into the future of 3D imaging with LUMI—where every layer counts.

Happy #LearningWednesday! ?? Today, we’re celebrating Alpenglow’s origins with a deep dive into the Nature Methods 2022 paper that laid the foundation for our HOTLS system. Today is a "look where we started" moment: “A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues.” https://lnkd.in/dtSgbv4t This work introduced HOTLS, a powerful imaging platform enabling high-speed, high-resolution 3D imaging. It revolutionized spatial biology with rapid whole-sample screening and targeted sub-micrometer imaging—unlocking unprecedented insights into large, intact specimens. "Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. "There is a need for a user-friendly system that can address imaging applications with varied requirements in terms of resolution, sample geometry, and compatibility with tissue-clearing protocols and sample holders of various refractive indices." "We designed a ‘hybrid’ OTLS microscope that is the first system to use a non-orthogonal dual-objective (NODO) configuration (for high-resolution imaging)." "For low-resolution imaging, a conventional ODO open-top system is integrated with the NODO system." "The hybrid microscope architecture consists of three objectives, positioned below the specimen, mounted into a monolithic imaging chamber that is filled with an interchangeable immersion medium." "By using a motorized stage, tiled imaging is possible with both paths over a large 12 x 7.5 x 1 cm (XYZ) imaging volume." "The ODO path provides fast meso-scale screening capabilities, and the NODO path enables targeted sub-micrometer imaging." "This large imaging volume accommodates multiple intact cleared organs and large tissue slabs mounted in an array of specimen holders." "Using the ODO imaging path, a mouse brain was rapidly screened in ~1 hour with isotropic ~2-μm voxels (~4-μm resolution), revealing brain-wide axonal projections." "A targeted region of interest around a cortical pyramidal neuron was then imaged at <0.5-um xy resolution using the NODO imaging path." "This imaging resolution is sufficient to discern spines and varicosities on individual dendrites and axons." "We used our hybrid OTLS system to study metastatic colonies from two cancer cell lines (MDA-231 and OS-RC-2) throughout intact mouse brains." "While past studies have relied on laborious and time-consuming experiments with manual transfer of individual specimens between different microscope systems, we analyzed six intact mouse brains in a single imaging session without manually removing or remounting the specimens." "Our hybrid OTLS system provides a unique combination of versatility and performance necessary to satisfy the diverse requirements of a growing number of cleared-tissue imaging applications." Text references are from the bioRxiv version.

? Discover Cutting-Edge 3D Imaging Over Coffee at ABRF 2025! Join us at ABRF 2025 in Las Vegas to explore the groundbreaking capabilities of our 3D i? Hybrid Open-Top Light-Sheet (HOTLS) Microscope. ?? March 25-26 ?? Las Vegas – ABRF Conference Let's discuss how this technology can advance your work over coffee. Drop a comment or DM to schedule a time or reserve your slot here: https://lnkd.in/d3a-4Pj6 Discover how the 3D i? HOTLS Microscope can transform your research with its seamless transition between whole-sample and high-resolution scans. Our instrument is designed for comprehensive 3D imaging of biological specimens, offering features such as: ?? Open-Top Design – Easily access samples with flexible holders for various sizes ??? Dual Optical Paths – Rapid Scout imaging & detailed Zoom imaging ?? Large Scan Area – Capture complete 3D images up to 12 cm x 7.5 cm x 1 cm #ABRF2025 #3DImaging #Microscopy #LifeSciences

Happy #FluorescenceFriday, everyone! Today, we are thrilled to showcase how advanced 3D imaging transforms our understanding of melanoma. By leveraging high-quality staining techniques, we’re moving beyond flat, 2D slides to capture the full spatial context of human melanoma samples. In our latest 3D video, observe: ?? CD45 – highlighting immune cells ?? Neutrophil Elastase – marking neutrophils ?? TO-PRO-3 – illuminating nuclei Each cell lights up like a star, revealing intricate spatial interactions that were once hidden. This depth of data not only enhances our insights but also sets a new standard in melanoma research. Proud to be part of a journey where the best imaging leads to the best insights! #3DImaging #SpatialBiology #Fluorescence #MelanomaResearch #InnovationInScience

Yesterday, we made a deep dive into the recent publication: “AI-driven 3D Spatial Transcriptomics.” https://lnkd.in/dxddspzE If we sparked your curiosity, join us for "Dinner with Data: The AI Revolution in 2D and 3D Pathology" on March 23, 2025, at Eddie Merlot’s Steakhouse in Boston. Many see data as just numbers. ? They overlook its power to change lives. ? They view AI as just a tool, not a revolution. ? They fail to realize how it can reshape pathology. At this special event, Dr. Andrew H. Song of Harvard Medical School will explore groundbreaking AI applications in pathology, including: ?? Weakly Supervised Machine Learning – Analyzing pathology samples with minimal labeled data. ?? Predictive Analytics in 2D & 3D Pathology – Enhancing disease diagnosis and prognosis. ?? AI in Pathology Workflows – Identifying high-risk disease features and guiding treatment decisions. ?? Advancements in 3D Imaging – Technologies like Optical Tissue Clearing for deeper tissue insights. This dinner is more than a meal—it’s a glimpse into the future of pathology. Join us to see how AI is transforming diagnosis and patient care. RSVP here: https://lnkd.in/d87FCyXr

Happy #LearningWednesday! Let’s review the recent and excellent pre-print: “AI-driven 3D Spatial Transcriptomics”, co-led by Cristina Almagro Pérez and Andrew H. Song. They describe VORTEX, an AI framework that predicts 3D transcriptomic profiles from minimal 2D data, offering faster, more cost-effective tissue analysis with improved scalability. https://lnkd.in/dVJn2bB6 "A comprehensive three-dimensional (3D) map of tissue architecture and gene expression is crucial for illuminating the complexity and heterogeneity of tissues across diverse biomedical applications." "However, most spatial transcriptomics (ST) approaches remain limited to two-dimensional (2D) sections of tissue." "Recent three-dimensional (3D) pathology studies, fueled by substantial advances in high-resolution 3D tissue imaging modalities such as micro computed tomography (microCT) or open-top light-sheet microscopy, showed that 3D morphological characterization can lead to better patient prognostication or cancer biomarker discovery." "While promising, in situ approaches remain limited in terms of capture area and depth, and require long processing times." "Serial section-based approaches provide discontinuous coverage along the axial dimension (i.e., 2.5D ST characterization) of thick tissues." "Such approaches are impractical for scaling to whole-volume transcriptomic profiling in terms of cost and effort." "We present VOlumetrically Resolved Transcriptomics EXpression (VORTEX), an AI framework that leverages 3D tissue morphology and minimal 2D ST to predict volumetric 3D ST." "VORTEX presents a fundamentally different mechanism for 3D ST prediction from other frameworks." "Existing works meticulously align multiple 2D tissue sections with 2D ST measurements from the same volume to construct a 2.5D ST heatmap." "Obtaining a 3D or 2.5D ST profile of a sample still results in high costs and turnaround time from having to sequence a large number of tissue sections." " VORTEX operates on continuous 3D tissue morphology as input, based on the underlying morphomolecular links learned by the models." "VORTEX can provide 3D ST for each volume with orders of magnitude less cost and time because it requires significantly fewer ST measurements from a VOI for fine-tuning." "Fine-tuning the model on a single 2D ST capture area from the VOI can help predict the ST profile for any other tissue regions outside the capture area, across the plane and at varying depths." "Incorporating depth context enhances the predictive performance, suggesting that the 3D context provides more morphological cues for predicting accurate transcriptomics expression." "VORTEX enables the integration and reuse of morphomolecular traits both across and within the samples, leveraging 3D tissue morphology as the common binding factor." Demo: https://lnkd.in/ejRtqNXv Blog post from Cristina Almagro Pérez: https://lnkd.in/eYQzpyPk Previous Cell paper from Dr. Song: https://lnkd.in/eBvrVf-3

?? Pathology is evolving—are you keeping up? AI is transforming pathology, and at USCAP 2025, you have the chance to hear from one of the pioneers leading the charge. Dr. Andrew H. Song (Harvard Medical School) will break down how weakly supervised machine learning is unlocking scalable, high-accuracy predictions in 2D and 3D pathology. This is the future of smarter diagnostics and personalized treatment. Connect with fellow experts, challenge the status quo, and engage in high-impact discussions over a gourmet dinner. ?? Eddie Merlot’s Steakhouse, Boston ?? March 23, 2025 | 6:00 PM ? Complimentary for Pharma, Biotech, and Academic professionals Seats are limited—don’t miss out! Reserve your spot now. ?? RSVP here: https://lnkd.in/d87FCyXr Stay tuned to discover more about the recent work of Dr. Andrew H. Song tomorrow on?#LearningWednesday! * H&E image is an example animation of OTLS (1um/voxel) core needle biopsy (prostate cancer) from Dr. Song blog post: https://lnkd.in/dy4GV4hd #USCAP2025 #Pathology #AIinHealthcare #3DPathology #Biotech #Innovation

?? Kicking off an exciting #AAD conference with a stunning #FluorescenceFriday! Feast your eyes on this full 3D video of a hair follicle (from bulb to shaft! ??????)—where every detail comes to life. ?? See the hair plexus— the dense network of sensory nerve fibers stained with PGP9.5 (green). These nerves stand out beautifully, while cell nuclei (TO-PRO-3) in red define the full hair structure. Why does 3D imaging matter? Because it reveals everything—structure, measurements, and insights critical for research and clinical pipeline advancements. ?? The future of hair biology is here. Let’s chat at AAD! → https://lnkd.in/dVfCA55h Where could you apply this tech? Drop your thoughts below! ?? #Innovation #3DImaging #HairBiology #AAD2025

Alpenglow Biosciences and Incyte Corporation to Present 3D Imaging Research at Society for Investigative Dermatology 2025 We are pleased to share that our collaborative research with Incyte Corporation has been accepted for an ePoster presentation at the Society for Investigative Dermatology (SID) 2025 Annual Meeting. ?? Presentation Title: "3D imaging elucidates neuroimmune dynamics in atopic dermatitis skin treated with ruxolitinib cream." ?? Authors: Angelina Volkova, PhD; Chifei Sun, MSc; Heather Bullins, HT, AAS; Konstantin Popovic, PhD; Shikhar Dhingra, MSc; Nathan Grant, MSc; Caleb Stoltzfus, PhD; Nicholas Reder, MD, MPH; Cynthia Timmers, PhD; Susan Smith, PhD ?? Presentation Details: ?? Session Title: ePoster Talks (Session 2) – Clinical Research: Interventional Research ?? Session Date & Time: May 9, 2025, from 4:45 PM to 5:45 PM ?? Room: Sapphire E/F ?? Presentation Time: 5:40 PM – 5:45 PM Led by Incyte Corporation, this research investigates the effects of 1.5% ruxolitinib cream on the neuroimmune landscape in atopic dermatitis (AD) using biopsy samples from the SCRATCH-AD study. Alpenglow Biosciences provided advanced 3D imaging and machine learning-driven analysis, providing a detailed analysis of nerve volume, immune cell distribution, and epidermal remodeling in response to treatment. Partnering with Incyte on this study underscores the power of cutting-edge 3D imaging in dermatological research, applying cutting-edge 3D imaging technology to provide an unprecedented view of spatial relationships within the skin microenvironment. Using Alpenglow’s proprietary Aurora 3Di light-sheet microscopy and advanced 3D analysis workflows, this study showcases how high-resolution, volumetric imaging can capture entire tissue structures in ways that traditional 2D methods cannot. By enabling a more comprehensive and unbiased evaluation of cellular organization, nerve-immune interactions, and tissue architecture, 3D imaging offers new opportunities to deepen our understanding of skin diseases and therapeutic responses. We are honored to support Incyte’s research in advancing treatment options for atopic dermatitis, a chronic and burdensome disease affecting millions worldwide. Through this collaboration, we strive to advance scientific innovation that translates into meaningful clinical impact, ultimately improving the lives of those affected by AD. We look forward to presenting this exciting work at SID 2025 and engaging with the dermatology community on the impact of 3D imaging in clinical applications. To learn more about Alpenglow Biosciences, Inc., visit our website https://lnkd.in/eGEM5gFJ.