Addgene

Looking to do some base editing? Check out our updated CRISPR 101 post "Cytosine and Adenine Base Editors." https://hubs.la/Q036vfp-0

Class 1 CRISPR systems are the most abundant in bacterial and archaeal immune systems but are often overlooked due to the multi-Cas Cascade complex. Read about the powerful Class 1 system Cascade-Cas3 and see the benefits in our latest blog post! https://hubs.la/Q03693Vn0

Addgene's Antibodies 101 eBook is a great resource for scientists using antibodies to study proteins. It contains a comprehensive introduction to antibodies as tools, as well as practical advice for common antibody applications like flow cytometry, western blots, IHC, ELISAs, and more. Check it out below! https://hubs.la/Q0362BRF0

C. acnes is the most abudant human skin microbe but.. can we make it the smartest? ??? Our latest Cell Press #CellSystems study shows how we engineered Cutibacterium acnes to actively protect the skin and developed a genetic toolkit to unlock its potential for microbiome-based therapies. ??? ?? Why C. acnes? Although some strains are linked to acne, C. acnes sticks when applied topically, has low turnover, is genetically stable, and lives in the very interesting hair follicle where the skin is the thinnest —making it an ideal chassis for synthetic biology applications. ?? What we built: ? Genetic toolbox – promoters, RBSs, terminators, inducible systems (available in Addgene and SEVA Plasmids!) ? Biocontainment strategies – to ensure safe potential skin application ? Potential therapeutic strain – secreting antioxidants to reducing UV-induced oxidative stress ?? Read the full study [Link in the comments] Thank you all people involved that made the article possible! Thank you Marc Güell for your mentorship, Javier Santos Moreno for your being my lab buddy all these years and the rest of the team at the #SynbioLab (Nil Campamà Sanz, Lorena Toloza Maturana, Cristóbal Parra Cid) in Universitat Pompeu Fabra PRBB (Barcelona Biomedical Research Park) for your help an support!! Also kudos to all the amazing SKINDEV_EU EIT - European Institute of Innovation and Technology for your contributions Ellen van den Bogaard Patrick Jansen from Radboudumc and I?vara Aor Rodrigo Ledesma-Amaro, PhD from Imperial College London #SyntheticBiology #Microbiome #CRISPR #Dermatology #Biotechnology --------------- ?? C. acnes es el microbio más abundante de la piel humana, pero… ?podemos hacerlo más inteligente? ??? En nuestro último estudio en Cell Press #CellSystems, hemos ingenierizado Cutibacterium acnes para que proteja activamente la piel y desarrollado un kit genético que desbloquea su potencial para terapias basadas en el microbioma. ? ?? ?Por qué C. acnes? Aunque algunas cepas están asociadas al acné, C. acnes se mantiene en la piel tras su aplicación, tiene baja renovación, es genéticamente estable y vive en el folículo piloso, donde la piel es más delgada—lo que lo convierte en un chasis ideal para aplicaciones de biología sintética. ?? ?Qué hemos desarrollado? ? Kit genético – promotores, RBSs, terminadores y sistemas inducibles (?disponibles en Addgene y SEVA Plasmids!) ? Estrategias de biocontención – para garantizar una aplicación segura en la piel ? Cepa terapéutica potencial – secretando antioxidantes para reducir el estrés oxidativo inducido por UV ?? ?? Lee el estudio completo [Enlace en comentarios] ? Gracias a todas las personas que hicieron posible este trabajo! #BiologíaSintética #Microbioma #CRISPR #Dermatología #Biotecnología

We’ve long believed biological discovery needs stronger antibody characterization standards. Our friends at YCharOS Inc./The Neuro (Montreal Neurological Institute-Hospital), Addgene and Abcam recently published consensus protocols for knockout cell-line-based antibody characterization that are sure to improve performance comparisons in western, IP and IF. Check out their work in Nature Protocols!

For researchers in many countries, ordering rabies and VSV-G plasmids is now easier and faster! Learn more in our recent blog post: https://hubs.la/Q035snDS0

Addgene has been in our Watertown, MA offices for six years now, and we are so proud of the many ways our employees have sought to share our science with our local community. Recently, Addgenie Alyssa joined the Watertown Little Local Conversations podcast to discuss her scientific journey, working at Addgene, and the volunteer work she and her coworkers do at the local high school. You can listen to it below: https://hubs.la/Q0351T2-0

CRISPR screens are a valuable part of the molecular biology toolbox. Lentiviral vectors are a popular method for delivering CRISPR libraries but may not always be the best option. Learn about the benefits of using AAV vectors in our newest blog post! https://hubs.la/Q0351WJg0

It can be hard to keep up with the new in-stock viral vectors in Addgene's repository. But our "New Viral Vectors" blog post can help. Click the link below to see the 49 new viral vectors we've added over the past three months. https://hubs.la/Q034chX90

Viral Vectors can be difficult to use, but Addgene's Viral Vectors 101 eBook can help! Our newly released second edition has more helpful content, more useful images, and increased accessibility for all. Find it here: https://hubs.la/Q02GPtX90