How can you optimize HPLC parameters for food analysis?

1 Choose the right column

The column is the heart of the HPLC system, where the separation of the analytes occurs. The column type, size, and material affect the resolution, efficiency, and selectivity of the separation. You need to choose a column that matches the polarity, molecular weight, and stability of your analytes, as well as the compatibility with the mobile phase and the detector. For example, if you want to analyze polar compounds, such as sugars or organic acids, you might use a reversed-phase column with a hydrophobic stationary phase and a polar mobile phase. On the other hand, if you want to analyze non-polar compounds, such as lipids or vitamins, you might use a normal-phase column with a polar stationary phase and a non-polar mobile phase.

3 Select the appropriate detector

The detector is the device that measures the signal of the analytes as they elute from the column. The detector type, wavelength, and sensitivity affect the accuracy, precision, and specificity of the detection. You need to select a detector that suits the nature, concentration, and response of your analytes, as well as the compatibility with the mobile phase and the column. For example, if you want to detect analytes that absorb ultraviolet or visible light, you might use a UV-Vis detector with a suitable wavelength and sensitivity. On the other hand, if you want to detect analytes that fluoresce or emit light, you might use a fluorescence or chemiluminescence detector with a suitable excitation and emission wavelength and sensitivity.

5 Here’s what else to consider

This is a space to share examples, stories, or insights that don’t fit into any of the previous sections. What else would you like to add?