Image analysis reproducibility is the ability to obtain the same or similar results from the same or equivalent data and methods by the same or different analysts. However, there are many factors that can affect the outcome of an image analysis project, such as the source, format, quality, or preprocessing of the images, the choice, implementation, or parameterization of the algorithms or models, the interpretation, presentation, or comparison of the results, and the human error, bias, or variation of the analysts. To improve image analysis reproducibility, it is recommended to use standardized or open formats, protocols, or platforms for storing, sharing, or accessing images and metadata; consistent or documented methods, tools, or libraries for processing, analyzing, or visualizing images and results; rigorous or validated methods, metrics, or benchmarks for evaluating, testing, or verifying images and results; and comprehensive or clear documentation, reports, or publications for describing, explaining, or citing images and results.