The last CSS best practice for animations and transitions is to consider the accessibility and usability of your web design. Animations and transitions can create visually appealing effects, but they can also cause problems for some users. To avoid causing discomfort or distraction, you should provide an option to disable or reduce animations and transitions, such as using the prefers-reduced-motion media query or a toggle button. Additionally, you should avoid animations or transitions that are too fast, too slow, too frequent, or too complex. It is also important to use animations or transitions that are meaningful and relevant, not just decorative or arbitrary, as they can help convey information, feedback, or context. Finally, you should test your animations or transitions on different devices, browsers, and screen sizes to ensure they do not affect the functionality, readability, or responsiveness of your web design.