What can you do to prevent pipette errors?

1 Choose the right pipette

The first step to prevent pipette errors is to choose the right pipette for your task. You should consider the volume range, accuracy, precision, and compatibility of the pipette with your samples and reagents. For example, you should use a fixed-volume pipette for repetitive measurements of the same volume, and a variable-volume pipette for different volumes. You should also avoid using a pipette at the extremes of its volume range, as this can compromise its accuracy and precision. Additionally, you should check the compatibility of the pipette materials with your samples and reagents, as some substances can corrode or damage the pipette components.

3 Control environmental conditions

The third step to prevent pipette errors is to control environmental conditions. You should be aware of how temperature, humidity, air pressure, and altitude can affect the volume and density of your samples and reagents, and adjust your pipette settings accordingly. You should also avoid exposing your pipette to direct sunlight, heat sources, or drafts, as these can cause evaporation or condensation in the tip or the barrel. Moreover, you should keep your pipette clean and dry, and store it in a safe and stable place when not in use.

4 Calibrate your pipette regularly

The fourth step to prevent pipette errors is to calibrate your pipette regularly. You should follow the manufacturer's recommendations and your laboratory's protocols for pipette calibration, which usually involve measuring the weight of water dispensed by the pipette at different volumes and comparing it to the expected values. You should also use a calibrated balance, distilled water, and a suitable container for pipette calibration, and perform it under controlled environmental conditions. If your pipette shows signs of inaccuracy or imprecision, you should recalibrate it or send it for service.

5 Maintain your pipette properly

The fifth step to prevent pipette errors is to maintain your pipette properly. You should perform routine maintenance tasks for your pipette, such as cleaning, lubricating, and replacing worn or damaged parts. You should also inspect your pipette regularly for any signs of leakage, corrosion, or malfunction, and report any issues to your laboratory manager or technician. Additionally, you should keep a record of your pipette's calibration and maintenance history, and follow the manufacturer's warranty and service guidelines.

6 Train and educate your staff

The sixth step to prevent pipette errors is to train and educate your staff. You should ensure that your staff are competent and confident in using your pipette, and that they follow the standard operating procedures and quality control measures for your laboratory. You should also provide them with regular feedback, refresher courses, and updates on the latest pipetting techniques and technologies. Furthermore, you should encourage them to share their experiences, challenges, and best practices with their colleagues, and to seek help or advice when needed.