What are the best practices for storing laboratory tools during PCR?

PCR (polymerase chain reaction) is a highly sensitive technique used in molecular biology to amplify specific DNA sequences. To ensure accurate and reliable results, it's crucial to properly store laboratory tools used during the process. Here are some best practices to follow: Preparation: Designated workspaces: Separate areas for pre-PCR and post-PCR activities help minimize contamination. Use dedicated tools for each area to prevent carryover. Labeling: Clearly label all tools, reagents, and samples to avoid mix-ups. Personal protective equipment (PPE): Wear gloves, lab coats, and eye protection throughout the experiment, changing them frequently when switching between areas.

Optimize the PCR facility by specifying separate pre-PCR and post-PCR areas with specific equipment. Clearly label equipment, dispose of trash properly, and wear gloves, lab coats, and eye protection. Regularly changing protective gear helps reduce the risk of contamination.

Storing pipettes and tips: Clean and dry environment: Store pipettes and tips in a clean, dry cabinet away from direct sunlight and heat sources, which can degrade plastics and affect accuracy. Minimize contamination: Avoid touching the tips with your fingers or any other surfaces. Use filter tips to prevent aerosols from entering the pipette. Calibration and maintenance: Regularly calibrate your pipettes according to the manufacturer's instructions to ensure accurate volume dispensing. Check for leaks or damage before each use.

Store pipette and tips in a clean, dry place away from sunlight and heat. Avoid touch tips and use filter tips to remove aerosols. Calibrate pipettes regularly, check for leakage damage, and ensure accurate fluid transfer during PCR.

Store tubes and tops in a cool, dark place such as the refrigerator to ensure identity of DNA sample and PCR reagent. Use sterile DNase-free tubes, avoid unnecessary openings and tightly cover tubes. Clear color or code helps to distinguish samples from raw materials during storage.

Maintain the productivity of the PCR reagent by storing it in a cool, dark place, preferably in a refrigerator. Follow the manufacturer’s storage instructions, and prevent repeated freeze-thaw. Separate the reagents into small pieces and use them as soon as possible to ensure efficiency in the PCR reaction.

Ensure a contamination-free environment after PCR by washing instruments with a 10% detergent solution or DNA decontamination solution. Rinse with distilled water and dry with a non-staining cloth. Dispose of used items in a biohazard container for better waste management.

Storing tubes and plates: Sealed containers: Keep tubes and plates containing samples or reagents sealed with caps or lids to prevent evaporation and contamination. Temperature control: Store samples and reagents at the appropriate temperature recommended by the manufacturer. DNA, for example, is typically stored at -20°C. Organization: Use racks or holders to organize tubes and plates for easy identification and retrieval.

Follow the golden rule of PCR:?DO NOT bring any reagents, equipment, or pipettes used in a post-PCR area back to the pre-PCR area.?This even goes for your lab notebook and pens. Label pre- and post-PCR items, so it's easy to tell where they belong.