Before you write your subject line, you need to know who you are writing to. What are their pain points, goals, interests, and preferences? How do they communicate and what tone do they respond to? Use these insights to tailor your subject line to your audience and segment them based on their behavior, demographics, or other criteria. For example, if you are sending a newsletter to your subscribers, you might use a friendly and casual tone, while if you are sending a sales pitch to a potential client, you might use a more professional and persuasive tone.