To use network segmentation and virtualization effectively, you should follow some best practices that can help you design, implement, and maintain your network topology and architecture. For instance, plan and map your network segments and virtual networks based on your business goals, security requirements, and network functions. Additionally, define and apply consistent and granular policies and controls such as access lists, firewalls, encryption, authentication, and monitoring. Moreover, it's important to test and validate your network segments and virtual networks before deploying them to ensure they work as intended without introducing any vulnerabilities or conflicts. Finally, review and update your network segments and virtual networks regularly to keep up with the evolving threats, technologies, and regulations.