How can you guarantee pipette reliability?

1 Choose the right pipette

The first step to ensure pipette reliability is to choose the right pipette for your application. You should consider the volume range, accuracy, precision, and compatibility of the pipette with the samples and reagents you are working with. For example, if you need to measure very small volumes, you should use a micropipette rather than a graduated pipette. You should also check the specifications and certificates of the pipette manufacturer and supplier, and make sure they meet the standards and requirements of your laboratory.

2 Follow the user manual

The second step to guarantee pipette reliability is to follow the user manual carefully. The user manual contains important information and instructions on how to use, clean, store, and troubleshoot the pipette. You should read the user manual before using the pipette for the first time, and refer to it whenever you have any doubts or issues. You should also follow the recommendations on how to handle and operate the pipette, such as how to hold it, how to adjust the volume, how to aspirate and dispense the liquid, and how to change the tips.

3 Perform regular checks

The third step to ensure pipette reliability is to perform regular checks on the pipette performance and function. You should check the pipette visually for any signs of damage, wear, or contamination, and inspect the tips for any cracks, leaks, or residues. You should also check the pipette functionally by performing a gravimetric test, which involves weighing the liquid dispensed by the pipette and comparing it with the expected value. You should perform this test at least once a month, or more frequently if you use the pipette often or for critical applications.

4 Calibrate the pipette

The fourth step to guarantee pipette reliability is to calibrate the pipette periodically or whenever needed. Calibration is the process of adjusting the pipette to ensure that it delivers the correct volume within the specified accuracy and precision. You should calibrate the pipette according to the manufacturer's instructions or the laboratory's protocol, using a calibrated balance and distilled water. You should also document the calibration results and keep a record of the pipette history. You should calibrate the pipette at least once a year, or more often if you notice any changes or deviations in the pipette performance.

5 Maintain the pipette

The fifth step to ensure pipette reliability is to maintain the pipette properly. Maintenance involves cleaning, disinfecting, lubricating, and repairing the pipette as needed. You should clean the pipette regularly with a soft cloth and a mild detergent, and disinfect it with an appropriate solution if you work with biological or hazardous materials. You should lubricate the pipette parts with a suitable grease or oil to prevent friction and corrosion. You should also repair the pipette if it is damaged or malfunctioning, and replace any worn or broken parts.