Wastewater treatment is the process of converting wastewater into a usable or safe form. It typically involves physical, chemical, and biological methods to reduce solids, organic matter, nutrients, pathogens, and toxic substances. Biotechnology can be used to enhance the biological methods of wastewater treatment by introducing microorganisms, enzymes, or plants to degrade pollutants. For instance, activated sludge is a popular biological method that uses a combination of microorganisms and oxygen to break down organic matter and nutrients in wastewater. The microorganisms are then removed from the treated water by sedimentation or filtration. Additionally, anaerobic digestion is another biological method that uses bacteria without oxygen to convert organic matter into biogas. This process reduces sludge volume and greenhouse gas emissions. Lastly, phytoremediation is a biological method that uses plants to absorb and degrade pollutants in wastewater. Plants can also provide oxygen, shade, and habitat for aquatic life. Phytoremediation can be used for wetlands, ponds, or constructed wetlands.